Main Title |
Teratogen Metabolism: Thalidomide Activation is Mediated by Cytochrome P-450. |
Author |
Braun, A. G. ;
Harding, F. A. ;
Weinreb, S. L. ;
|
CORP Author |
Massachusetts Inst. of Tech., Cambridge. Dept. of Applied Biological Sciences.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1986 |
Report Number |
EPA/600/J-86/089; |
Stock Number |
PB86-208527 |
Additional Subjects |
Toxicology ;
Metabolism ;
Reprints ;
Thalidomide ;
Metabolites ;
Teratogenesis
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB86-208527 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
7p |
Abstract |
A metabolite of thalidomide generated by hepatic microsomes inhibited the attachment of tumor cells to concanavalin A-coated polyethylene. Evidence that metabolite formation is mediated by microsomal cytochrome P-450 is presented. Microsomes incubated with thalidomide underwent a type I spectral shift. Metabolite formation was reduced or eliminated by carbon monoxide, SKF-525A, metyrapone, and N-octylamine. Superoxide dismutase treatment had no effect. Metabolite formation required microsomes and NADPH and was dependent on the length of 37 C incubation. The metabolite could be isolated by successive hexane and chloroform extractions. It is likely the inhibitory thalidomide metabolite was generated by a minor cytochrome P-450 species. Whether the thalidomide metabolite is involved in the drug's teratogenic activity remains to be shown. |
Supplementary Notes |
Pub. in Toxicology and Applied Pharmacology, v82 n1 p175-179 Jan 86. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Teratogen Metabolism: Thalidomide Activation is Mediated by Cytochrome P-450. |
PUB Date Free Form |
c1986 |
Category Codes |
6T; 57Y |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
622718371 |
Origin |
NTIS |
Type |
CAT |