Main Title |
Practical capillary electrophoresis / |
Author |
Weinberger, Robert.
|
Publisher |
Academic Press, |
Year Published |
1993 |
OCLC Number |
27222974 |
ISBN |
0127423559 (acid-free paper); 9780127423555 (acid-free paper) |
Subjects |
Capillary electrophoresis ;
Metodos instrumentais de analise (quimica) ;
Electrophoresis--methods
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
EKBM |
QP519.9.C36W45 1993 |
|
Research Triangle Park Library/RTP, NC |
03/29/2010 |
EMBM |
QP519.9.C36W45 1993 |
|
NRMRL/GWERD Library/Ada,OK |
09/16/1994 |
|
Collation |
xv, 312 p. : ill. ; 24 cm. |
Notes |
Includes bibliographical references and index. |
Contents Notes |
Introduction -- Electrophoresis -- Microchromatographic separation methods -- Capillary electrophoresis -- Historical perspective -- Instrumentation -- Sources of information on HPCE -- Capillary electrophoresis: a family ot techniques -- Basic concepts -- Electrical conduction in fluid solution -- Language of electrophoresis -- Electroendoosmosis -- Efficiency -- Resolution -- Joule heating -- Optimizing the voltage and temperature -- Capillary diameter and buffer ionic strength -- Optimizing the capillary length -- Buffers -- Capillaries -- Capillary zone electrophoresis -- Mobility -- Buffer additives -- Solute-wall interactions -- Bandbroadening -- Applications -- Methods development -- Capillary isoelectric focusing -- Capillary coatings and electroosmotic flow -- Performing a run -- Focusing and mobilization problems -- Applications -- Size separations in capillary gels and polymer networks -- Separation mechanism -- Materials for CGE -- Applications -- Pulsed-field capillary electrophoresis -- Future of CGE -- Capillary isotachophoresis -- Separation mechanism -- Instrumental aspects -- Buffer selection. (cont) Advantages and problems with CITP -- Applications -- Electrokinetic capillary chromatography -- Micelles -- Separation mechanism -- Sources of bandbroadening -- Selecting the electrolyte system -- Elution range of MECC -- Alternative surfactant systems -- Applications and methods development -- Cyclodextrins -- Chiral recognition -- Optimization -- Capillary electrochromatography -- Electroosmotic pump -- Bandbroadening -- Applications -- Electrochromatography in perspective -- Injection -- Volumetric constraints on injection size -- Performing an injection and run -- Injection techniques -- Injection problems and solutions -- Alternative injection schemes -- Stacking -- Other enrichment techniques -- Detection -- On-capillary detection -- Detection problem -- Classes of detectors -- Limits of detection -- Bandbroadening -- Absorption detection -- Fluorescence detection -- Indirect detection -- Mass spectrometry -- Other detection techniques -- Fraction collection -- Putting it all together -- Selecting the mode of HPCE -- Requirements for robust separations. (cont) Realistic compromises -- Linear dynamic range -- Sample preparation -- Quantitative analysis -- Correction factors -- Special topics -- Field effect electroendoosmosis -- Ultrafast separations -- HPCE on a chip -- Multiple capillary systems -- Gradient and programmed systems -- Two-dimensional systems -- Immunologic trace enrichment. |
Place Published |
Boston |
PUB Date Free Form |
c1993 |
BIB Level |
m |
Cataloging Source |
OCLC/T |
LCCN |
92043461 |
OCLC Time Stamp |
20100322154959 |
Language |
eng |
Origin |
OCLC |
Type |
CAT |
OCLC Rec Leader |
03996cam 2200481 a 45020 |