| Main Title |
Development of Methods to Measure Virus Inactivation in Fresh Waters. |
| Author |
Ward, R. L. ;
Winston, P. E. ;
|
| CORP Author |
James N. Gamble Inst. of Medical Research, Cincinnati, OH.;Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1985 |
| Report Number |
EPA/600/J-85/353; |
| Stock Number |
PB86-175486 |
| Additional Subjects |
Fresh water ;
Viruses ;
Water pollution control ;
Enteroviruses ;
Measurement ;
Reprints ;
Inactivation
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB86-175486 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
7p |
| Abstract |
The study concerns the identification and correction of deficiencies in methods used to measure inactivation rates of enteric viruses seeded into environmental waters. It was found that viable microorganisms in an environmental water sample increased greatly after addition of small amounts of nutrients normally present in the unpurified seed virus preparation. This burst of microbial growth was not observed after seeding the water with purified virus. The use of radioactively-labeled poliovirus revealed that a high percentage of virus particles, sometimes >99%, were lost through adherence to containers, especially on less turbid waters. This effect was partially overcome by the use of polypropylene containers and by the absence of movement during incubation. Adherence to containers clearly demonstrated the need for labeled viruses to monitor losses in this type of study. Loss of viral infectivity in samples found to occur during freezing was avoided by addition of broth. |
| Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v50 n5 p1144-1148 Nov 85. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Development of Methods to Measure Virus Inactivation in Fresh Waters. |
| PUB Date Free Form |
c1985 |
| Category Codes |
6M; 57K; 68D |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
614222750 |
| Origin |
NTIS |
| Type |
CAT |
