| Main Title |
Stress Ethylene: A Bioassay for Rhizosphere-Applied Phytotoxicants. |
| Author |
Rodecap, Kent D. ;
Tingey, David T. ;
|
| CORP Author |
Corvallis Environmental Research Lab., OR. |
| Year Published |
1981 |
| Report Number |
EPA-600/J-81-523; |
| Stock Number |
PB82-169186 |
| Additional Subjects |
Ethylene ;
Toxicology ;
Plants(Botany) ;
Bioassay ;
Responses ;
Stems(Botany) ;
Reprints ;
Phytotoxicants
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB82-169186 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
11p |
| Abstract |
A bioassay for rhizosphere-applied phytotoxicants was developed and evaluated with a broad range of chemicals. Test substances were applied to the rhizosphere of whole, intact bush bean plants (Phaseolus vulgaris L. cv. Bush Blue Lake 290) grown in a solid support medium and the resultant ethylene production was measured to detect the presence of phytotoxic materials. The beans were encapsulated in plastic bags for 2 hrs following treatment and then incubated for 24 hrs in the dark. Ethylene and ethane accumulating within the bags were quantified via gas-solid chromatography. The application of various concentration of inorganic and organic chemicals induced various responses. No single equation adequately described the dose-response curves; therefore, a critical value (a statistically significant increase in stress ethylene production) was computed for each test substance. A phytotoxic-response threshold for each test substance was defined as the test substance concentration that caused ethylene production to exceed its respective critical-value concentration. Based on threshold concentrations determined by analysis of stress ethylene production, the relative phytotoxicity rankings of the inorganic test substances were: CdCl2 > CuCl2 > Pb(C2H302)2 > LiCl, while those of the organic test substances were 2,4-D esters < paraquat dicloride < 1-butanol < 2-propanone < 2-propanol. Both stress ethylene and ethane production were nonresponsive to hydrogen-ion concentration (measured before application) over a broad range. |
| Supplementary Notes |
Pub. in Environmental Monitoring and Assessment 1, p119-127 1981. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Stress Ethylene: A Bioassay for Rhizosphere-Applied Phytotoxicants. |
| PUB Date Free Form |
1981 |
| Category Codes |
6T; 6C; 57Y; 57C |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/02 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
323811586 |
| Origin |
NTIS |
| Type |
CAT |
