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Main Title Final report on development of test for determining an aerobic biodegradation potential /
Author Shelton, D. R. ; Tiedje, J. M.
Other Authors
Author Title of a Work
Tiedje, J. M.
CORP Author Michigan State Univ., East Lansing. Dept. of Crop and Soil Sciences.;Environmental Protection Agency, Washington, DC. Office of Pesticides and Toxic Substances.
Publisher Office of Pesticides and Toxic Substances, U.S. Environmental Protection Agency,
Year Published 1981
Report Number EPA/560/R-81/013; EPA-68-01-5043; EPA-560/5-81-013
Stock Number PB84-166495
Subjects Sewage--Purification--Biological treatment ; Biodegradation
Additional Subjects United States--Laws, etc--Toxic Substances Control Act ; Anaerobic processes ; Biodeterioration ; Organic compounds ; Environmental surveys ; Sludge disposal ; Solid waste disposal ; Industrial wastes ; Metabolism ; Oxidation reduction reactions ; Sampling ; Toxic substances ; Sewage sludge ; Gas production rates
Library Call Number Additional Info Location Last
NTIS  PB84-166495 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 92 pages ; 28 cm.
Abstract This project was for the purpose of developing and evaluating a suitable screening-level laboratory method for the evaluation of the potential that an organic compound might be susceptible to anaerobic biodegradation (methanogenesis) in the environment. As it developed, the project focused on a method under consideration by the American Society for Testing and Materials (ASTM) and the report contains several recommendations as appropriate, for the modification of the draft ASTM method. Overall, the report, with the recommended modifications, consists of a single-lab validation of the basic method. The method also appears as one of the EPA Office of Toxic Substances Chemical Fate Test Guidelines. In the method, a chemically defined anaerobic medium containing resazurin, as an oxidation/reduction indicator, and 10 primary anaerobic digestor sludge are dispensed in 100-mL portions into serum bottles. Selected bottles are supplemented with test substance at a concentration equivalent to 50 mg/L as organic carbon. Gas production is measured at appropriate intervals. The extent of biodegradation is determined by comparing gas production from blank control bottles and bottles containing the test substance.
Notes "September 1981." Chiefly tables. "Contract no. 68-01-5043." Includes bibliographical references (pages 36-37). Microfiche.
Place Published {Washington, D.C.} :
Corporate Au Added Ent Michigan State University. Dept. of Crop and Soil Sciences. ; United States. Environmental Protection Agency. Office of Pesticides and Toxic Substances.
Title Ser Add Ent Technical directive (United States. Environmental Protection Agency. Office of Pesticides and Toxic Substances) ;
PUB Date Free Form {1981}
Series Title Untraced Technical directive / Office of Pesticides and Toxic Substances, U.S., Environmental Protection Agency ; 16
Ti Tra Differently Development of test for determining an aerobic biodegradation. Aerobic biodegradation.
NTIS Prices PC A05/MF A01
BIB Level m
Cataloging Source OCLC/T
OCLC Time Stamp 20040105112622
Language eng
Origin NTIS
OCLC Rec Leader 01725nam 2200373Ka 45020