| Main Title |
Stage-Specific Damage to Synaptonemal Complexes and Metaphase Chromosomes Induced by X Rays in Male Mouse Germ Cells. |
| Author |
Backer, L. C. ;
Sontag, M. R. ;
Allen, J. W. ;
|
| CORP Author |
Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Duke Univ. Medical Center, Durham, NC.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. |
| Publisher |
c1991 |
| Year Published |
1991 |
| Report Number |
EPA-68-01-4456; EPA/600/J-91/040; |
| Stock Number |
PB91-182238 |
| Additional Subjects |
Synaptonemal complex ;
Chromosome aberrations ;
Metaphase ;
X rays ;
Spermatozoa ;
Mice ;
Mutagens ;
Deoxyribonucleic acids ;
Cell cycle ;
Electron microscopy ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-182238 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
12p |
| Abstract |
Synaptonemal complexes (SCs) reveal mutagen-induced effects in germ cell meiotic chromosomes. The study was aimed at characterizing relationships between SC and metaphase I chromosome damage following radiation exposure at various stages of spermatogenesis. Male mice were irradiated with doses of 0, 2, or 4 Gy, and spermatocytes were harvested at times consistent with earlier exposures as spermatogonial stem cells, preleptotene cells (premeiotic DNA synthesis), or meiotic prophase cells. After stem-cell exposure, twice as many rearrangements were observed in SCs as in metaphase I chromosomes. Irradiation during premeiotic DNA synthesis resulted in dose-related increases in SC breakage and rearrangements (including novel forms) and in metaphase chromosomal aberrations. Following prophase exposure, various types and levels of SC and metaphase damage were observed. Irradiation of zygotene cells led to high frequencies of chromosome multivalents in metaphase I without a correspondingly high level of damage in preceding prophase SCs. Thus, irradiation of premeiotic and meiotic cells results in variable relationships between SC and metaphase chromosome damage. |
| Supplementary Notes |
Pub. in Radiation Research, v125 n1 p187-196 Jan 91. Prepared in cooperation with Duke Univ. Medical Center, Durham, NC. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Stage-Specific Damage to Synaptonemal Complexes and Metaphase Chromosomes Induced by X Rays in Male Mouse Germ Cells. |
| Category Codes |
57V; 57F |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
117926995 |
| Origin |
NTIS |
| Type |
CAT |
