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OLS Field Name OLS Field Data
Main Title Effects of 2,4-Dichlorophenol, a Metabolite of a Genetically Engineered Bacterium, and 2,4-Dichlorophenoxyacetate on Some Microorganism-Mediated Ecological Processes in Soil.
Author Short, K. A. ; Doyle, J. D. ; King, R. J. ; Seidler, R. J. ; Stotzky, G. ;
CORP Author Corvallis Environmental Research Lab., OR. ;NSI Technology Services Corp., Corvallis, OR. Environmental Research Lab. ;New York Univ., NY. Dept. of Biology. ;Michigan Univ., Ann Arbor. Medical School.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-91/144;
Stock Number PB91-219022
Additional Subjects Genetic engineering ; Pseudomonas ; Soil microbiology ; Terrestrial ecosystems ; Pesticides ; Soil contamination ; Mass fragmentography ; Metabolism ; Glucose ; Plasmids ; Fungi ; Toxicity ; Kinetics ; Reprints ; Dichlorophenols ; Dichlorophenoxyacetates
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NTIS  PB91-219022 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 11/26/1991
Collation 9p
Abstract A genetically engineered microorganism, Pseudomonas putida PP0301 (pR0103), and the plasmidless parent strain, PP0301, were added at approximately ten to the seventh CFU/g of soil amended with 500 ppm of 2,4-dichlorophenoxyacete (2,4-D) (500 microgram/g). The degradation of 2,4-D and the accumulation of a single metabolite, identified by gas chromatography-mass spectrophotometry as 2,4-dichlorophenol (2,4-DCP), occurred only in soil inoculated with PP0301 (pR0103), wherein 2,4-DCP accumulated to >70 ppm for 5 weeks and the concentration of 2,4-D was reduced to <100 ppm. Coincident with the accumulation of 2,4-DCP was a >400-fold decline in the numbers of fungal propagules and a marked reduction in the rate of CO2 evolution, whereas 2,4-D did not depress either fungal propagules or respiration of the soil microbiota. 2,4-DCP did not appear to depress the numbers of total heterotrophic, sporeforming, or chitin-utilizing bacteria. (Copyright (c) 1991, American Society for Microbiology.)
Supplementary Notes Pub. in Applied and Environmental Microbiology, v57 n2 p412-418 Feb 91. Prepared in cooperation with NSI Technology Services Corp., Corvallis, OR. Environmental Research Lab., New York Univ., NY. Dept. of Biology, and Michigan Univ., Ann Arbor. Medical School.
NTIS Title Notes Journal article.
Title Annotations Reprint: Effects of 2,4-Dichlorophenol, a Metabolite of a Genetically Engineered Bacterium, and 2,4-Dichlorophenoxyacetate on Some Microorganism-Mediated Ecological Processes in Soil.
Category Codes 68E; 57K; 57F; 57B
NTIS Prices PC A02/MF A01
Primary Description 600/02
Document Type NT
Cataloging Source NTIS/MT
Control Number 126923588
Origin NTIS
Type CAT