| Main Title |
Cloning and Characterization of a Chromosomal DNA Region Required for Growth on 2,4,5-T by 'Pseudomonas cepacia' AC1100. |
| Author |
Haugland, R. A. ;
Sangodkar, U. M. X. ;
Sferra, P. R. ;
Chakrabarty, A. M. ;
|
| CORP Author |
Illinois Univ. at the Medical Center, Chicago. Dept. of Microbiology and Immunology.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.;National Inst. of Environmental Health Sciences, Research Triangle Park, NC. |
| Publisher |
c1991 |
| Year Published |
1991 |
| Report Number |
NIEHS-ES04050-06; EPA/600/J-93/214; |
| Stock Number |
PB93-204972 |
| Additional Subjects |
Pseudomonas cepacia ;
Bacteria DNA ;
Molecular cloning ;
2-4-5-trichlorophenoxyacetic acid ;
Growth ;
Mutations ;
Nucleic acid hybridization ;
Cosmid ;
Genetic complementation test ;
Bacterial genes ;
Gene expression ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-204972 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
11p |
| Abstract |
A series of spontaneous 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) nonmetabolizing mutants of Pseudomonas cepacia AC1100 were characterized to be defective in either 2,4,5-T uptake or conversion of the compound to 2,4,5-trichlorophenol (2,4,5-TCP). Two of these mutants, RHC22 and RHC23, were complemented for growth on 2,4,5-T using an AC1100 genomic library constructed in the cosmid vector pCP13. Recombinant cosmids isolated from the complemented mutants contained a 27.5-kb insert which frequently underwent various-sized deletions in Escherichia coli. Hybridization studies showed the DNA to be of chromosomal origin and totally deleted in RHC22, RHC23 and other similar mutants. Complementation analyses of RHC22 with a series of subcloned fragments and spontaneously deleted derivatives of the recombinant cosmid pRHC21 showed the 2,4,5-T(tft) genes to occur within an 8.9-kb region. Pseudomonas aeruginosa cells transformed with the DNA acquired the ability to convert 2,4,5-T to 2,4,5-TCP. The genetic determinant for this function was further localized within a 3.7-kb region. The DNA, in the absence of other sequences from the 8.9-kb tft gene region allowed RHC22 cells to metabolize 2,4,5-T, but at low rates which were insufficient to support growth. (Copyright (c) 1991 Elsevier Science Publishers B.V.) |
| Supplementary Notes |
Pub. in Gene, v11 p66-73, 1991. See also PB90-100520. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab., and National Inst. of Environmental Health Sciences, Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Cloning and Characterization of a Chromosomal DNA Region Required for Growth on 2,4,5-T by 'Pseudomonas cepacia' AC1100. |
| Category Codes |
57F; 57K; 57B |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/14 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
323828364 |
| Origin |
NTIS |
| Type |
CAT |
