| Main Title |
DNA Adduct Formation, Metabolism, and Morphological Transforming Activity of Aceanthrylene in C3H10T1/2CL8 Cells. |
| Author |
Nesnow, S. ;
Ross, J. ;
Mohapatra, N. ;
Gold, A. ;
Sangaiah, R. ;
|
| CORP Author |
Baylor Coll. of Medicine, Houston, TX. ;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.;Environmental Protection Agency, Research Triangle Park, NC. |
| Publisher |
c1989 |
| Year Published |
1989 |
| Report Number |
EPA-R-811817, EPA-68-02-4031; EPA/600/J-89/055; |
| Stock Number |
PB90-103458 |
| Additional Subjects |
Toxicity ;
Metabolism ;
Aromatic polycyclic hydrocarbons ;
Thin layer chromatography ;
Reprints ;
DNA damage ;
Mutagenicity tests ;
Aceanthrylene ;
Cultured cells
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB90-103458 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
15p |
| Abstract |
Aceanthrylene (ACE), a cyclopenta-fused polycyclic aromatic hydrocarbon (CP-PAH) related to anthracene, has been studied for its ability to be metabolized, to form DNA adducts, and to morphologically transform C3H101/2CL8 mouse embryo fibroblasts in culture. ACE is metabolized by C3H101T1/2 cells to ACE-1,2-dihydrodiol (the cyclopenta-ring dihydrodiol) at a rate of 450 pmoles ACE-1,2-dihydrodiol formed/hr10 sup 6 cells. ACE-7,8-dihydrodiol and ACE-9,10-dihydrodiol, identified as major Aroclor-1254 induced rat liver microsomal metabolites from their UV, NMR, and mass spectral data, were not identified in incubations of C3H10T1/2 cells with ACE. ACE-DNA adducts in C3H10T1/2 cells were isolated, separated, identified, and quantitated using the 32p-postlabeling method. ACE forms four major adducts and each was identified as ACE-1,2-oxide/2'-de-oxyguanosine adducts. ACE-DNA adduct persistence and repair were evaluated in C3H10T1/2 cells using a hydroxyurea block after ACE treatment. ACE-DNA adducts were not repaired under the conditions used in the morphological transformation studies. Thus, ACE provides an interesting example of a mutagenic PAH which is metabolized by C3H10T1/2 cells to active intermediates, forms relatively stable and persistent 2'-deoxyguanosine adducts in C3H10T1/2 cells, and yet induces no detectable morphological transforming activity under the experimental conditions used. |
| Supplementary Notes |
Pub. in Jnl. of Mutation Research, v222 p223-235, 1989. Prepared in cooperation with North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering. Sponsored by Environmental Protection Agency, Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: DNA Adduct Formation, Metabolism, and Morphological Transforming Activity of Aceanthrylene in C3H10T1/2CL8 Cells. |
| Category Codes |
57Y; 57F |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
200/04 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
935216098 |
| Origin |
NTIS |
| Type |
CAT |
