Main Title |
Monoclonal Antibodies to Chlorinated Dibenzo-p-Dioxins. |
Author |
Kennel, S. J. ;
Mason, G. ;
Safe, S. ;
|
CORP Author |
Oak Ridge National Lab., TN. Biology Div. ;Texas A and M Univ., College Station.;Environmental Research Lab.-Duluth, MN. |
Publisher |
c1986 |
Year Published |
1986 |
Report Number |
EPA-810995; EPA/600/J-86/536; |
Stock Number |
PB90-265109 |
Additional Subjects |
Antigens ;
Mice ;
Reprints ;
Monoclonal antibodies ;
Trichlorodibenzodioxin ;
Substrate specificity ;
Hybridomas ;
Radioimmunoassay ;
Bovine serum albumin ;
Binding sites
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB90-265109 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
5p |
Abstract |
A thyroglobulin conjugate of dioxin (thyroglobulin-2 adipamide, 3,7,8-trichlorodibenzo-p-dioxin) (TG-TCDD) was used to immunize BALB/c mice. Hybridomas were produced by cell fusion between immune spleen cells and mouse myelomas SP2/O, P3, or NS1. In order to screen the thousands of resultant cultures for production of MoAb, a rapid, solid phase radioimmunoassay for antibody to dioxins was developed. This involved attaching bovine serum albumin coupled with trichlorodibenzo-p-dioxin (BSA-TCDD) to polystyrene plates to be used as a solid phase target antigen for reaction with MoAb. Fourteen hybridomas were identified that produced MoAb reacting with BSA-TCDD but not with BSA alone. Antibodies were tested for binding to BSA-aniline to eliminate those with limited binding specificity. Initial studies indicated that most MoAbs bound BSA-aniline as well as BSA-TCDD. More detailed analyses indicated that while most MoAbs showed some reaction with BSA-aniline, two showed preferential binding to BSA-TCDD of more than 200 fold whereas rabbit antisera demonstrated only a 5-fold discrimination. MoAb 391-1B was purified from mouse ascites fluid and after radioiodination, was tested for direct binding to BSA-TCDD or BSA-aniline. (125)I MoAb showed no significant binding to BSA-aniline while demonstrating high binding to BSA-TCDD (Ka = 4.5 x 10 to the eighth/mole). |
Supplementary Notes |
Pub. in Chemosphere, v15 n9-12 p2007-2010 1986. Prepared in cooperation with Texas A and M Univ., College Station. Sponsored by Environmental Research Lab.-Duluth, MN. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Monoclonal Antibodies to Chlorinated Dibenzo-p-Dioxins. |
Category Codes |
57J; 68G |
NTIS Prices |
PC A01/MF A01 |
Primary Description |
600/03 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
029924814 |
Origin |
NTIS |
Type |
CAT |