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Main Title Root-Mycocosm for Growing Ectomycorrhizal Hyphae Apart from Host Roots while Maintaining Symbiotic Integrity (Journal Version).
Author Rygiewicz, P. T. ; Miller, S. L. ; Durall, D. M. ;
CORP Author Corvallis Environmental Research Lab., OR. ;Oregon State Univ., Corvallis. Dept. of Forest Science.;National Science Foundation, Washington, DC.;Pacific Northwest Forest and Range Experiment Station, Corvallis, OR. Forestry Sciences Lab.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/233; NSF-BSR85-05975 ;NSF-BSR83-06181;
Stock Number PB89-142715
Additional Subjects Fungi ; Cultures(Biology) ; Gravimetric analysis ; Plants(Botany) ; Plant growth ; Reprints ; Mycorrhizas ; Roots
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NTIS  PB89-142715 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 5p
Abstract A culture system is described to grow mycorrhizal plants which allows experimental measurements to be made on mycorrhizae, and a portion of intact ectomycorrhizal fungi while in symbiosis, but growing apart from the rooting medium and host roots. A portion of the extramatrical hyphae is kept apart from the rooting medium by a restrictive passageway between the root and fungal chambers. Fungal hyphae grow out of the root chamber onto pre-weighted glass fiber filter paper for gravimetric estimation of the hyphal mass in the fungal chamber. The pieces of the modular Root-Mycocosm can be assembled in various configurations. Ponderosa pine (pinus ponderosa Laws.) seedlings were inoculated with Hebeloma crustuliniforme Quel in either commercial-vermiculite inoculum or in plastic growth-pouches and grown in the Root-Mycocosm. Hyphae were allowed to grow into the fungal chambers and after four weeks, amounted to 5.5 + or - 0.81 SE and 6.4 + or - 1.5 SE mg for pouch and commercial inoculum techniques, respectively. (Copyright 1988 Kluwer Academic Publishers.)
Supplementary Notes Pub. in Plant and Soil, v109 p281-284 1988. Prepared in cooperation with Oregon State Univ., Corvallis. Dept. of Forest Science. Sponsored by National Science Foundation, Washington, DC., and Pacific Northwest Forest and Range Experiment Station, Corvallis, OR. Forestry Sciences Lab.
NTIS Title Notes Journal article.
Title Annotations Reprint: Root-Mycocosm for Growing Ectomycorrhizal Hyphae Apart from Host Roots While Maintaining Symbiotic Integrity (Journal Version).
Category Codes 57C; 57B
NTIS Prices Not available NTIS
Primary Description 600/02
Document Type NT
Cataloging Source NTIS/MT
Control Number 911822674
Origin NTIS
Type CAT