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OLS Field Name OLS Field Data
Main Title PCR Primers Specific for Detection of a Rat Repetitive Sequence.
Author Mass, M. J. ; Roop, B. C. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Carcinogenesis and Metabolism Branch.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/440;
Stock Number PB93-141398
Additional Subjects Polymerase chain reaction ; Nucleic acid repetitive sequences ; Molecular cloning ; Rats ; Mice ; Cultured cells ; Transfection ; DNA probes ; Reprints ;
Holdings
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NTIS  PB93-141398 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/12/1993
Collation 4p
Abstract Identification of a new oncogene is often accomplished by transfection of genomic DNA of interest into a host of a different species that can respond to the altered phenotype. We report a method for tracking and identifying potential genes of interest that are derived from rat DNA. We used polymerase chain reaction to amplify specifically the rat long-interspersed repetitive sequences in mouse cells that were transfected with rat DNA.
Supplementary Notes Pub. in BioTechniques, v13 n5 p676-678 Nov 92.
NTIS Title Notes Journal article.
Title Annotations Reprint: PCR Primers Specific for Detection of a Rat Repetitive Sequence.
Category Codes 57F
NTIS Prices PC A01/MF A01
Primary Description 600/10
Document Type NT
Cataloging Source NTIS/MT
Control Number 307031468
Origin NTIS
Type CAT