Main Title |
Growth of 'Legionella pneumophila' in Continuous Culture. |
Author |
Berg, J. D. ;
Hoff, J. C. ;
Roberts, P. V. ;
Matin, A. ;
|
CORP Author |
Environmental Protection Agency, Cincinnati, OH. Water Engineering Research Lab. ;Rio Linda Chemical Co., Sacramento, CA. ;Stanford Univ., CA. |
Year Published |
1985 |
Report Number |
EPA/600/J-85/092; |
Stock Number |
PB85-238475 |
Additional Subjects |
Bacteria ;
Cultures(Biology) ;
Growth ;
Culture media ;
Reprints ;
Legionella pneumophila
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB85-238475 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
5p |
Abstract |
A method was developed to grow Legionella pneumophila in continuous culture. A chemostat was used to simulate nutrient-limited, sub-maximal growth in the natural environmental and to provide a precisely-controlled growth regime. Cultures grew under forced aeration under conditions yielding up to 38% saturation of dissolved oxygen; supplemental CO2 (5%) at the same gas flow rates as ambient air had no effect on culture growth. Pleomorphiam was observed during growth under all conditions. Pigment was produced only at D < 0.03 h-1. Catalase was produced at higher growth rates, but not at higher temperatures. The pathogenicity was unaffected by altering either the growth rate or growth temperature. |
Supplementary Notes |
Prepared in cooperation with Rio Linda Chemical Co., Sacramento, CA. and Stanford Univ., CA. |
Availability Notes |
Pub. in Applied and Environmental Microbiology 49, n6 p1534-1537 Jun 85. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Growth of 'Legionella pneumophila' in Continuous Culture. |
PUB Date Free Form |
c1985 |
Category Codes |
6M; 57K |
NTIS Prices |
Not available NTIS |
Primary Description |
600/14 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
531124988 |
Origin |
NTIS |
Type |
CAT |