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Main Title Growth of 'Legionella pneumophila' in Continuous Culture.
Author Berg, J. D. ; Hoff, J. C. ; Roberts, P. V. ; Matin, A. ;
CORP Author Environmental Protection Agency, Cincinnati, OH. Water Engineering Research Lab. ;Rio Linda Chemical Co., Sacramento, CA. ;Stanford Univ., CA.
Year Published 1985
Report Number EPA/600/J-85/092;
Stock Number PB85-238475
Additional Subjects Bacteria ; Cultures(Biology) ; Growth ; Culture media ; Reprints ; Legionella pneumophila
Library Call Number Additional Info Location Last
NTIS  PB85-238475 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 5p
Abstract A method was developed to grow Legionella pneumophila in continuous culture. A chemostat was used to simulate nutrient-limited, sub-maximal growth in the natural environmental and to provide a precisely-controlled growth regime. Cultures grew under forced aeration under conditions yielding up to 38% saturation of dissolved oxygen; supplemental CO2 (5%) at the same gas flow rates as ambient air had no effect on culture growth. Pleomorphiam was observed during growth under all conditions. Pigment was produced only at D < 0.03 h-1. Catalase was produced at higher growth rates, but not at higher temperatures. The pathogenicity was unaffected by altering either the growth rate or growth temperature.
Supplementary Notes Prepared in cooperation with Rio Linda Chemical Co., Sacramento, CA. and Stanford Univ., CA.
Availability Notes Pub. in Applied and Environmental Microbiology 49, n6 p1534-1537 Jun 85.
NTIS Title Notes Journal article.
Title Annotations Reprint: Growth of 'Legionella pneumophila' in Continuous Culture.
PUB Date Free Form c1985
Category Codes 6M; 57K
NTIS Prices Not available NTIS
Primary Description 600/14
Document Type NT
Cataloging Source NTIS/MT
Control Number 531124988
Origin NTIS
Type CAT