| Main Title |
Use of a Novel Plasmid to Monitor the Fate of a Genetically Engineered 'Pseudomonas putida' Strain. |
| Author |
Genthner, F. J. ;
Campbell, R. P. ;
Pritchard, P. H. ;
|
| CORP Author |
Environmental Research Lab., Gulf Breeze, FL. ;Technical Resources, Inc., Gulf Breeze, FL. |
| Publisher |
c1992 |
| Year Published |
1992 |
| Report Number |
EPA/600/J-93/066; |
| Stock Number |
PB93-169001 |
| Additional Subjects |
Pseudomonas putida ;
Genetic engineering ;
Antibiotic resistance ;
Plasmids ;
Deoxyribonucleic acids ;
Nucleic acid hybridization ;
Aquatic microbiology ;
Biodeterioration ;
Phenotype ;
Growth ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-169001 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
Plasmid pSI30 was constructed to increase the sensitivity of detection of a genetically engineered microorganism (GEM) and its recombinant DNA in environmental samples. The broad host-range, mobilizable plasmid contained chlorocatechol (clc) degradative genes, antibiotic resistance genes (ampicillin and kanamycin), and a fragment of eukaryotic DNA. The clc genes encode enzymes that convert 3-chlorocatechol to maleylacetic acid permitting the host, Pseudomonas putida RC-4, to grow on 3-chlorobenzoate. The catabolic phenotype was exploited using enrichment procedures to detect RC-4(pSI30) cells, freeliving in the water column or when irreversibly bound to surfaces. The eukaryotic DNA sequence provided a unique target allowing positive identification by DNA:DNA hybridization. In flow-through microcosms, RC-4(pSI30), undetectable as freeliving cells, was found by enrichment as irreversibly bound sessile forms. These experiments revealed the stability of pSI30 and its utility in a combination detection system for tracking the survival of a GEM and its DNA in environmental samples. |
| Supplementary Notes |
Pub. in Molecular Ecology 1, p137-143 1992. See also PB91-206870. Prepared in cooperation with Technical Resources, Inc., Gulf Breeze, FL. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Use of a Novel Plasmid to Monitor the Fate of a Genetically Engineered 'Pseudomonas putida' Strain. |
| Category Codes |
57K; 57F |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/04 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
314124355 |
| Origin |
NTIS |
| Type |
CAT |
