Main Title |
Regulation of Gene Expression Molecular Mechanisms / [electronic resource] : |
Type |
EBOOK |
Author |
Perdew, Gary H.
|
Other Authors |
|
Publisher |
Humana Press, |
Year Published |
2007 |
Call Number |
QD415-436 |
ISBN |
9781597452281 |
Subjects |
Life sciences ;
Human genetics ;
Toxicology ;
Biochemistry
|
Internet Access |
|
Collation |
IX, 333 p. online resource. |
Notes |
Due to license restrictions, this resource is available to EPA employees and authorized contractors only |
Contents Notes |
Gene Expression Control At The Mrna Level -- Overview -- Messenger RNA Accumulation -- Transcript Profiling -- Transcriptional Regulation of Gene Expression -- Posttranscriptional Processing of Messenger RNA -- Regulation Of Protein Levels And Transcription Factor Function -- Overview -- Protein Synthesis and Turnover -- Proteomics -- Determination of Protein-Protein Interactions and the Motifs That Mediate Them -- Posttranslational Modifications -- Dissection of Signaling Pathways -- Use Of Transgenic And Knockout Mice To Study Gene Regulation -- Overview -- Production of Transgenic Animals -- Knockout Animals -- Isolation of Cells and Cell Lines From Transgenic and Knockout Animals. Regulation of Gene Expression: Molecular Mechanisms presents a comprehensive overview of methods and approaches for characterizing mechanisms of gene regulation. The text is appropriate both as a graduate textbook and a standard laboratory reference and provides the essential groundwork for an advanced understanding of the various mechanisms that may result in altered activity of a specific cell protein. Each of three sections explores mechanisms of gene regulation and expression, and presents methods and protocols for achieving specific experimental goals. Part I focuses on approaches for studying control of mRNA expression and determining target genes for a given transcription copy. Part II outlines the methods for determining how proteins can regulate each other by mediating synthesis, degradation, protein-protein interactions, and posttranslational modification. Part III explores how gene targeting techniques in mice can provide insight into protein function. This volume provides a clear, concise overview of the protocols and techniques used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. |
Place Published |
Totowa, NJ |
Corporate Au Added Ent |
SpringerLink (Online service) |
Host Item Entry |
Springer eBooks |
PUB Date Free Form |
2007 |
BIB Level |
m |
Medium |
computer |
Content |
text |
Carrier |
online resource |
Cataloging Source |
OCLC/T |
OCLC Time Stamp |
20141123022019 |
Language |
eng |
Origin |
SPRINGER |
Type |
EBOOK |
OCLC Rec Leader |
03377nam a22004815i 45 |