Full Record Display for the EPA National Library Catalog


Main Title Fertilization by Sperm Microinjection and Zona-Drilling: Methods.
Author Perreault, S. D. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1988
Report Number EPA/600/D-88/095;
Stock Number PB88-211776
Additional Subjects Reproduction(Biology) ; Fertility ; Sperm microinjection ; Zona drilling method ; Sperm ; Eggs
Library Call Number Additional Info Location Last
NTIS  PB88-211776 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 30p
Abstract Successful microinjection of sperm or sperm nuclei into oocytes depends upon many factors. For example, injection needles must be large enough to pick up a sperm (nucleus) yet not so large as to kill the oocyte. The suction/pressure system should be very precisely controlled so as to minimize injection of excess fluid into the oocyte. As is true of other micromanipulation procedures, the microscope should provide a wide range of magnifications, long working distance, and a fixed stage; and, chambers must allow for easy addition/removal of oocytes with minimal disturbance of micropipet position. With all of these components operating together, the time required to inject small groups (or single) oocytes can be minimized thus avoiding, as much as possible, excessive oocyte aging and the deleterious effects of adverse environmental conditions outside of the incubator.
Supplementary Notes See also PB88-211784.
PUB Date Free Form May 88
Category Codes 57E#
NTIS Prices PC A03/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 821127490
Origin NTIS
Type CAT