Main Title |
Production of Monoclonal Antibodies to 'Legionella pneumophila' Serogroups 1 and 6. |
Author |
Para, M. F. ;
Plouffe, J. F. ;
|
CORP Author |
Ohio State Univ., Columbus. Div. of Infectious Diseases.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1983 |
Report Number |
EPA-R-812704; EPA/600/J-83/357; |
Stock Number |
PB87-170130 |
Additional Subjects |
Antigens ;
Lobar pneumonia ;
Serology ;
Immunology ;
Epidemiology ;
Isolation ;
Diffusion ;
Agglutination ;
Reprints ;
Legionella pneumophila ;
Legionnaires disease ;
Monoclonal antibodies
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB87-170130 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
8p |
Abstract |
To better define the surface antigens of Legionella pneumophila for clinical and experimental purposes, were produced monoclonal antibodies to L. pneumophila serogroups 1 and 6. Two hybridomas were produced in serogroup 1. One antibody, LP-I-17, recognized a serogroup-common antigen. The second antibody, LP-I-81, was specific for serogroup 1. Microagglutination assays of environmental and clinical isolates revealed a subgroup of serogroup 1 environmental isolates which were not agglutinated by LP-I-81. Immunodiffusion studies showed identity between the LP-I-81 antigen and the serogroup-specific antigen of serogroup 1 organisms. This antigen could be absorbed out of the serogroup 1 organism extract with LP-I-81-coated Staphylococcus aureus, leaving the serogroup-common antigens. Three hybridomas were produced to serogroup 6. All three produced antibodies which were serogroup 6 specific and agglutinated serogroup 6 bacteria. (Copyright (c) 1982, American Society for Microbiology.) |
Supplementary Notes |
Pub. in Jnl. of Clinical Microbiology, v18 n4 p895-900 Oct 86. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Production of Monoclonal Antibodies to 'Legionella pneumophila' Serogroups 1 and 6. |
PUB Date Free Form |
c1983 |
Category Codes |
57J; 57K; 57U |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
716918315 |
Origin |
NTIS |
Type |
CAT |