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OLS Field Name OLS Field Data
Main Title Hypochlorite-Serum Reaction Products Inhibit Porcine Vascular Endothelial Cell Growth in Culture.
Author Papa, L. R. ; McCauley, P. T. ;
CORP Author Health Effects Research Lab., Cincinnati, OH.
Year Published 1985
Report Number EPA/600/J-85/242;
Stock Number PB86-137072
Additional Subjects Toxicology ; Chlorination ; Inhibitors ; In vitro analysis ; Blood cells ; Growth ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB86-137072 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 14p
Abstract In vitro toxicity studies were initiated in order to determine if chlorination affects vascular endothelial cells. Twelfth to twentieth passage porcine aortic vascular endothelial cells (PAE) were grown to confluency and replated in the presence of complete media (Eagle's minimum essential media supplemented with 20% fetal bovine serum) which had been preincubated for 30 minutes with 15.0 mg/liter chlorine. During a 72-hour exposure period, control PAE cells grew to confluency, an increase of approximately 9 fold in the number of cells/plate. Those cells exposed to media preincubated with 15.0 mg/liter chlorine derived from sodium hypochlorite increased only 6 fold. There was no sign of cell killing, but an apparent inhibition of cell division. No effect was seen when either the amino acid or vitamin component of the complete media was reacted at the 15.0 mg dose level. However, when the serum component was preincubated with 15.0 mg chlorine/liter as sodium hypochlorite, an inhibition in growth rate similar to the complete media occurred.
Supplementary Notes Pub. in Artery 13, n1 p50-60 1985. Portions of this document are not fully legible.
NTIS Title Notes Journal article.
Title Annotations Reprint: Hypochlorite-Serum Reaction Products Inhibit Porcine Vascular Endothelial Cell Growth in Culture.
PUB Date Free Form 1985
Category Codes 6T; 57Y
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 605827070
Origin NTIS
Type CAT