Main Title |
In vitro Comparison of Rat and Chicken Brain Neurotoxic Esterase. |
Author |
Novak, R. ;
Padilla, S. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Northrop Services, Inc., Research Triangle Park, NC. |
Year Published |
1986 |
Report Number |
EPA/600/J-86/069; |
Stock Number |
PB86-213774 |
Additional Subjects |
Esterase ;
Toxicology ;
Brain ;
In vitro analysis ;
Rats ;
Chickens ;
Laboratory animals ;
Comparison ;
Reprints ;
Neurotoxicology
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB86-213774 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
10p |
Abstract |
A systematic comparison was undertaken to characterize neurotoxic esterase (NTE) from rat and chicken brain in terms of inhibitor sensitivities, pH optima, and molecular weights. Paraoxon titration of phenyl valerate (PV)-hydrolyzing carboxylesterased showed that rat esterases were more sensitive than chicken to paraoxon inhibition at concentrations less than micromole and superimposable with chicken esterases at concentrations of 2.5-1000 micromole. Mipafox titration of the paraoxon-resistant esterases at a fixed paraoxon concentration of 100 micromole (mipafox concentration: 0-1000 micromole) resulted in a mipatox 150 of 7.3 micromole for chicken brain NTE and 11.6 micromole for rat brain NTE. NTE(i.e., paraoxon-resistant, mipafox-sensitive esterase activity) comprised 80% of chicken and 60% of rat brain paraoxon-resistant activity with the specific activity of chicken brain NTE approximately twice that of rat brain NTE. The pH maxima for NTE from both species was similar showing broad, slightly alkaline optima from pH 7.9 to 8.6. (3H)Diisopropyl phosphorofluoridate (DFP)-labeled NTE from the brains of both species had an apparent mol wt of 160,000 measured by sodium dodecyl sulfate polyaacrylamide gel electrophoresis. |
Supplementary Notes |
Pub. in Fundamental and Applied Toxicology 6, n3 p464-471 Apr 86. Prepared in cooperation with Northrop Services, Inc., Research Triangle Park, NC. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: In vitro Comparison of Rat and Chicken Brain Neurotoxic Esterase. |
PUB Date Free Form |
c1986 |
Category Codes |
6T; 57Y |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
624112253 |
Origin |
NTIS |
Type |
CAT |