||Relationship between Tumorigenic Potency, Ki-ras Codon 12 Mutations, and DNA Adducts Induced by Cyclopenta(cd)pyrene.
Nesnow, S. ;
Ross, J. A. ;
Nelson, G. ;
Wilson, K. ;
Roop, B. C. ;
||Medical Coll. of Ohio at Toledo. Dept. of Pathology. ;Ohio State Univ., Columbus. Dept. of Preventive Medicine. ;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering. ;Integrated Lab. Systems, Research Triangle Park, NC.;Environmental Protection Agency, Research Triangle Park, NC. Carcinogenesis and Metabolism Branch.
DNA adducts ;
Ras genes ;
Pulmonary neoplasms ;
Dose-response relationships ;
Metabolic activation ;
Animal disease models ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Cyclopenta(cd)pyrene (CPP) was examined for its lung tumorigenic activity in strain A/J mice, for the formation and persistence of CPP-induced DNA adducts in lung tissue, and for its induction of mutations in the Ki-ras oncogene from CPP-induced turmors. CPP displayed high tumorigenic activity, including 97.7 lung adenomas/mouse at 200 mg/kg. Ki-ras codon 12 mutations in the DNA of induced tumors were: GGT --> CGT (50%); GGT --> GTT (15%); GGT --> TGT (25%); GGT --> GAT (10%). All DNA adducts in the lungs of CPP-treated mice were CPP-3,4-oxide derived and most were CPP-3,4-oxide-2'-deoxyguanosine adducts. CPP is highly tumorigenic in the strain A/J mouse lung adenoma model, being 5 times more active than benzo(a)pyrene. The increased activity of CPP may be related to the unique induction of the GGT --> CGT, Ki-ras codon 12 mutation. (Copyright (c) 1994 Gordon and Breach Science Publishers S.A.)
||Pub. in Polycyclic Aromatic Compounds, v6 p161-168 Aug 94. Prepared in cooperation with Ohio State Univ., Columbus. Dept. of Preventive Medicine., North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering., and Integrated Lab. Systems, Research Triangle Park, NC.
||%AUT:A. J. /Jeffers ;A. J. /Galati ;G. D. /Stoner ;R. /Sangaiah ;A. /Gold ;M. J. /Mass
|NTIS Title Notes
||Reprint: Relationship between Tumorigenic Potency, Ki-ras Codon 12 Mutations, and DNA Adducts Induced by Cyclopenta(cd)pyrene.
||57Y; 57F; 57E
||PC A02/MF A01