||Tracking Bacterial Pollution Sources in Hampton Harbor. A Final Report to the United States Environmental Protection Agency and the New Hampshire Estuaries Project/Office of State Planning.
S. H. Jones ;
||New Hampshire Univ., Durham. Dept. of Natural Resources.; New Hampshire Dept. of Environmental Services, Concord. Water Management Bureau.; New Hampshire Office of State Planning, Concord.; Environmental Protection Agency, Washington, DC.
Water pollution sources ;
Water sampling ;
Escherichia coli ;
Species diversity ;
Surface waters ;
Water pollution effects(Animals) ;
Water quality ;
New Hampshire ;
Laboratory tests ;
Hampton Harbor(New Hampshire)
||Some EPA libraries have a fiche copy filed under the call number shown.
||Fecal-borne microorganisms impact many shellfish-growing waters in coastal New Hampshire. Watersheds are often subject to fecal contamination by a variety of sources and efforts to improve water quality are often limited because of lack of information on which contaminant sources are most significant. Ribotyping and other microbial source tracking methods are useful new tools for providing information on the sources of fecal-borne bacterial contaminants in surface waters. New Hampshire has areas of abundant oyster (Crassostrea virginica) and clam (Mya arenaria) resources, the latter being most important in Hampton Harbor. In this study, Escherichia coli isolates (bacteria colonies) were obtained from water samples collected from ten sites in Hampton Harbor year-round during both dry and wet conditions. A library of known E. coli isolates was created from twenty different potential source species in the New Hampshire coastal watershed, including humans, livestock, pets, wildlife and avian species. The ribosomal RNA DNA of E. coli isolates was analyzed using ribotyping in which the patterns of ribosomal DNA were detected using chemiluminescence, then optimized and analyzed using GelCompar II software.
||Prepared in cooperation with New Hampshire Dept. of Environmental Services, Concord. Water Management Bureau. Sponsored by New Hampshire Office of State Planning, Concord. and Environmental Protection Agency, Washington, DC.
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