| Main Title |
(32)p-Adduct Assay: Principle and Applications to Carcinogen-Exposed Animal and Human DNA. |
| Author |
Gupta, R. C. ;
Sharma, S. ;
Earley, K. ;
Mohapatra, N. ;
Nesnow, S. ;
|
| CORP Author |
Baylor Coll. of Medicine, Houston, TX. ;M.D. Anderson Hospital and Tumor Inst., Houston, TX.;Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1987 |
| Report Number |
EPA/600/D-87/077; |
| Stock Number |
PB87-166948 |
| Additional Subjects |
Carcinogens ;
Deoxyribonucleic acids ;
Malignant neoplasms ;
Nucleoxides ;
Mutagens ;
Mutations ;
32 P labeling assay ;
DNA adducts
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB87-166948 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
18p |
| Abstract |
There is growing evidence that carcinogens initiate the malignant process via specific alterations in DNA structure, i.e., the covalent binding of carcinogens to DNA bases. Thus, carcinogen-DNA adducts represent as markers for tumor initiation. Several new techniques have been reported for detecting exceptionally small quantities of adducts without requiring test chemicals to be radioactive. These methods are based on specific antibodies, fluorescence spectra, electrophore labeling, and 32P incorporation into DNA constituents. Recent developments have indicated that the (32)P labeling method is capable of measuring a wide spectra of unusually low levels .10 to the tenth power nucleotides of DNA adducts induced by a vast majority of known aromatic/hydrophobic environmental carcinogens (Gupta, 1985). The paper reviews the underlying principle of the approach, as well as some recent applications. |
| Supplementary Notes |
Prepared in cooperation with M.D. Anderson Hospital and Tumor Inst., Houston, TX. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| PUB Date Free Form |
Mar 87 |
| Category Codes |
57F; 57; 57B; 68G |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
715626454 |
| Origin |
NTIS |
| Type |
CAT |
