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RECORD NUMBER: 43 OF 345

OLS Field Name OLS Field Data
Main Title Changes in In vitro Brain and Spinal Cord Protein Phosphorylation After a Single Oral Administration of Tri-o-Cresyl Phosphate to Hens.
Author Patton, S. E. ; Lapadula, D. M. ; O'Callaghan, J. P. ; Miller, D. B. ; Abou-Donia, M. B. ;
CORP Author Duke Univ. Medical Center, Durham, NC. Dept. of Pharmacology.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1985
Report Number EPA/600/J-85/431;
Stock Number PB86-224573
Additional Subjects Toxicology ; Protein ; In vitro analysis ; Brain ; Spinal cord ; Phosphorylation ; Reprints ; Neurotoxicity ; Tri-o-cresyl phosphate
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NTIS  PB86-224573 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 13p
Abstract The effect of a single oral 750 mg/kg dose of tri-o-cresyl phosphate (TOCP) on the endogenous phosphorylation of brain and spinal cord proteins was assessed in hens during the development of and recovery from delayed neurotoxicity. Crude membrane and cytosolic fractions were prepared from the brains and spinal cords of control and TOCP-treated hens at 1, 7, 14, 21, 35, and 55 days after treatment. Brain and spinal cord protein phosphorylation with (gamma-32P)ATP was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), autoradiography, and microdensitometry. TOCP administration conferred calcium and calmodulin dependence on the phosphorylation of a few brain cytosolic proteins and caused an increase in the phosphorylation of a number of other cytosolic and membrane proteins.
Supplementary Notes Pub. in Jnl. of Neurochemistry, v45 n5 p1567-1577 May 85. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Changes in In vitro Brain and Spinal Cord Protein Phosphorylation After a Single Oral Administration of Tri-o-Cresyl Phosphate to Hens.
PUB Date Free Form c1985
Category Codes 6T; 57Y
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 625520611
Origin NTIS
Type CAT