||Changes in In vitro Brain and Spinal Cord Protein Phosphorylation After a Single Oral Administration of Tri-o-Cresyl Phosphate to Hens.
Patton, S. E. ;
Lapadula, D. M. ;
O'Callaghan, J. P. ;
Miller, D. B. ;
Abou-Donia, M. B. ;
||Duke Univ. Medical Center, Durham, NC. Dept. of Pharmacology.;Health Effects Research Lab., Research Triangle Park, NC.
In vitro analysis ;
Spinal cord ;
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||The effect of a single oral 750 mg/kg dose of tri-o-cresyl phosphate (TOCP) on the endogenous phosphorylation of brain and spinal cord proteins was assessed in hens during the development of and recovery from delayed neurotoxicity. Crude membrane and cytosolic fractions were prepared from the brains and spinal cords of control and TOCP-treated hens at 1, 7, 14, 21, 35, and 55 days after treatment. Brain and spinal cord protein phosphorylation with (gamma-32P)ATP was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), autoradiography, and microdensitometry. TOCP administration conferred calcium and calmodulin dependence on the phosphorylation of a few brain cytosolic proteins and caused an increase in the phosphorylation of a number of other cytosolic and membrane proteins.
||Pub. in Jnl. of Neurochemistry, v45 n5 p1567-1577 May 85. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
|NTIS Title Notes
||Reprint: Changes in In vitro Brain and Spinal Cord Protein Phosphorylation After a Single Oral Administration of Tri-o-Cresyl Phosphate to Hens.
|PUB Date Free Form
||PC A02/MF A01