| Main Title |
Characterization of the Origins of Astrocyte Response to Injury Using the Dopaminergic Neurotoxicant, 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydrophyridine. |
| Author |
O'Callaghan, J. P. ;
Miller, D. B. ;
Reinhard., J. F. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Wellcome Research Labs., Research Triangle Park, NC. |
| Publisher |
c25 Jun 90 |
| Year Published |
1990 |
| Report Number |
EPA/600/J-90/231; |
| Stock Number |
PB91-117077 |
| Additional Subjects |
Toxicology ;
Nervous system ;
Nerve cells ;
Mice ;
Reprints ;
Astrocytes ;
Methyl tetrahydropyridines ;
Dopamine receptors ;
Glial fibrillary acidic protein ;
Radioimmunoassay ;
Tyrosine hydroxylase ;
Phosphoproteins ;
Mitogens ;
Interleukin-1
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-117077 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
The amount of glial fibrillary acidic protein (GFAP), an astrocyte protein, increases following injury of the CNS. A radioimmunoassay of GFAP was used to characterize the astrocytic response to injury resulting from exposure to the dopaminergic neurotoxicant, 1-methyl-1,2,3,6-tetrahydropyridine (MPTP). A single administration of MPTP to the C57BL/6 mouse resulted in more than a 3-fold increase in GFAP within 48 hours, followed by a decline to baseline at 3 weeks. A decrease in the amount of tyrosine hydroxylase (TH), a marker of nigrostriatal dopaminergic neurons, preceded the rise in GFAP. The concentration of DARPP-32, a phosphoprotein localized to striatal neurons receiving dopaminergic input, was not affected by MPTP. Protecting the dopaminergic neurons from the neurotoxic metabolite of MPTP, 1-methyl-4-phenylpyridinium (MPP+), either by blocking its formation or by preventing its uptake into dopamine neurons, completely blocked the increase in GFAP. Blood-borne or brain-derived interleukin 1 (IL-1), a known astrocyte mitogen, did not appear to mediate the effects of MPTP on GFAP. (Copyright (c) 1990 Elsevier Science Publishers B.V. (Biomedical Division)) |
| Supplementary Notes |
Pub. in Brain Research, v521 p73-80 1990. Prepared in cooperation with Wellcome Research Labs., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Characterization of the Origins of Astrocyte Response to Injury Using the Dopaminergic Neurotoxicant, 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydrophyridine. |
| Category Codes |
57Y; 57F |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
101123883 |
| Origin |
NTIS |
| Type |
CAT |
