| Main Title |
Glutathione Plus Cytosol- and Microsome-Mediated Binding of 1,2-Dichloroethane to Polynucleotides. |
| Author |
Lin, E. L. C. ;
Mattox, J. K. ;
Pereira, M. A. ;
|
| CORP Author |
Health Effects Research Lab., Cincinnati, OH. |
| Year Published |
1985 |
| Report Number |
EPA/600/J-85/139; |
| Stock Number |
PB85-249852 |
| Additional Subjects |
Nucleotides ;
Chemical bonds ;
Separation ;
Chromatographic analysis ;
Reprints ;
Ethane/dichloro ;
Microsomes ;
Glutathime ;
High performance liquid chromatography
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB85-249852 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
1,2-Dichloroethane-(1,2(14)C) was metabolized by rat liver microsomes to products that irreversibly bound polynucleotides. The polynucleotides were then enzymatically hydrolyzed and the products separated by HPLC equipped with an ODS or a SCX column. The products of microsome mediated binding were identified in the HPLC eluate as 1,N(6)-ethenoadenosine to polyadenylic acid, 3,N(sub 4)-ethenocytidine to polycytidylic acid, and two cyclic derivatives to polyguanylic acid. Chromatography by ODS-HPLC of the major peak from Sephadex G-25 indicated the presence of a GSH metabolite of 1,2-dichloroethane that does not contain a nucleoside. A similar hydrophilic peak was obtained from the hydrolysis products of polynucleotides from a glutathione plus cytosol incubations in which the polynucleotides instead of being added prior to the incubation were added after the incubation. The products of the glutathione plus cytosol metabolism of (14 sub c)-1,2-dichloroethane appear to be glutathione metabolites that were co-isolated with the polynucleotides rather than covalently bound adducts. In conclusion, covalently bound adducts were identified for microsome mediated binding of 1,2-dichloroethane to polynucleotides, while no evidence was obtained for glutathione plus cytosol mediated covalent binding to polynucleotides. |
| Supplementary Notes |
Pub. in Toxicology and Applied Pharmacology 78, p428-435 May 85. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Glutathione Plus Cytosol- and Microsome-Mediated Binding of 1,2-Dichloroethane to Polynucleotides. |
| PUB Date Free Form |
1985 |
| Category Codes |
6A; 7D; 57B; 99F |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
532621626 |
| Origin |
NTIS |
| Type |
CAT |
