| Main Title |
Growth of 'Legionella pneumophila' in Continuous Culture. |
| Author |
Berg, J. D. ;
Hoff, J. C. ;
Roberts, P. V. ;
Matin, A. ;
|
| CORP Author |
Environmental Protection Agency, Cincinnati, OH. Water Engineering Research Lab. ;Rio Linda Chemical Co., Sacramento, CA. ;Stanford Univ., CA. |
| Year Published |
1985 |
| Report Number |
EPA/600/J-85/092; |
| Stock Number |
PB85-238475 |
| Additional Subjects |
Bacteria ;
Cultures(Biology) ;
Growth ;
Culture media ;
Reprints ;
Legionella pneumophila
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB85-238475 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
5p |
| Abstract |
A method was developed to grow Legionella pneumophila in continuous culture. A chemostat was used to simulate nutrient-limited, sub-maximal growth in the natural environmental and to provide a precisely-controlled growth regime. Cultures grew under forced aeration under conditions yielding up to 38% saturation of dissolved oxygen; supplemental CO2 (5%) at the same gas flow rates as ambient air had no effect on culture growth. Pleomorphiam was observed during growth under all conditions. Pigment was produced only at D < 0.03 h-1. Catalase was produced at higher growth rates, but not at higher temperatures. The pathogenicity was unaffected by altering either the growth rate or growth temperature. |
| Supplementary Notes |
Prepared in cooperation with Rio Linda Chemical Co., Sacramento, CA. and Stanford Univ., CA. |
| Availability Notes |
Pub. in Applied and Environmental Microbiology 49, n6 p1534-1537 Jun 85. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Growth of 'Legionella pneumophila' in Continuous Culture. |
| PUB Date Free Form |
c1985 |
| Category Codes |
6M; 57K |
| NTIS Prices |
Not available NTIS |
| Primary Description |
600/14 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
531124988 |
| Origin |
NTIS |
| Type |
CAT |
