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Main Title Field Calibration of Soil Core Microcosms for Evaluating Fate and Effects of Genetically Engineered Microorganisms in Terrestrial Ecosystems.
Author Bolton, H. ; Fredrickson, J. K. ; Bentjen, S. A. ; Workman, D. J. ; Li, S. W. ;
CORP Author Battelle Pacific Northwest Labs., Richland, WA.;Corvallis Environmental Research Lab., OR.;Department of Energy, Washington, DC.
Publisher Apr 91
Year Published 1991
Report Number DE-AC06-76RLO-1830; EPA/600/3-91/041;
Stock Number PB91-216366
Additional Subjects Terrestrial ecosystems ; Soil microbiology ; Genetic engineering ; Pseudomonas ; Seasonal variations ; Field tests ; Temperature ; Soil water ; Microbial colony count ; Risk assessment ; Soil-core microcosms ; Streptomyces lividans
Library Call Number Additional Info Location Last
NTIS  PB91-216366 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 88p
Abstract Pacific Northwest Laboratory compared intact soil-core microcosms and the field for ecosystem structural and functional properties after the introduction of a model genetically engineered microorganism (GEM). The project used two distinct microbial types as model GEMs, Gram-negative Pseudomonas sp RC1, which was an aggressive root colonizer, and Gram-positive Streptomyces lividans TK24. The model GEMs were added to surface soil in separate studies throughout a ten month period. Also, RC1 was used in studies conducted during two consecutive field seasons to determine how year-to-year field variability influenced the calibration of microcosms with the field. The main conclusions of the research were that intact soil-core microcosms can be useful to simulate the field for studies of microbial fate and effects on ecosystem structural and functional properties.
Supplementary Notes Sponsored by Corvallis Environmental Research Lab., OR., and Department of Energy, Washington, DC.
Category Codes 57K; 57F; 48E
NTIS Prices PC A05/MF A01
Primary Description 600/02
Document Type NT
Cataloging Source NTIS/MT
Control Number 125534625
Origin NTIS
Type CAT