| Main Title |
Intraterminal Injection of Synapsin I or Calcium/Calmodulin-Dependent Protein Kinase II Alters Neurotransmitter Release at the Squid Giant Synapse. |
| Author |
Llinas, R. ;
McGuinness, T. L. ;
Leonard, C. S. ;
Sugimori, M. ;
Greengard, P. ;
|
| CORP Author |
Rockefeller Univ., New York. ;New York Univ. Medical Center, NY. ;Marine Biological Lab., Woods Hole, MA.;Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1985 |
| Report Number |
EPA-R-810608; EPA/600/J-85/457; |
| Stock Number |
PB87-145611 |
| Additional Subjects |
Neural transmission ;
Squids ;
Synapses ;
Laboratory animals ;
Experimentation ;
Synaptic vescicles ;
Protein phosphorylation ;
Synaptic transmission
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB87-145611 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
8p |
| Abstract |
Synapsin I and calcium/calmodulin-dependent protein kinase II were pressure-injected into the preterminal digit of the squid giant synapse to test directly the possible regulation of neurotransmitter release by these substances. Injection of dephosynapsin I decreased the amplitude and rate of rise of the postsynaptic potential, whereas injection of either phosphosynapsin I or heat-treated dephosphosynapsin I was without effect. Conversely, injection of calcium/calmodulin-dependent protein kinase II, which phosphorylates synapsin I on site II, increased the rate of rise and amplitude and decreased the latency of the postsynaptic potential. The effects of these proteins were observed without any detectable change in the initial phase of the presynaptic calcium current. A synapsin I-like protein and calcium/calmodulin-dependent protein kinase II were demonstrated by biochemical and immunochemical techniques to be present in squid nervous tissue. The data support the hypothesis that synapsin I regulates the availability of synaptic vesicles for release. (Copyright (c) 1985, Neurobiology.) |
| Supplementary Notes |
Pub. in Proceedings of National Academy of Science USA, Neurobiology, v82 p3035-3039 May 85. Prepared in cooperation with New York Univ. Medical Center, NY., and Marine Biological Lab., Woods Hole, MA. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| PUB Date Free Form |
c1985 |
| Category Codes |
57I; 95C; 57Z |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
712828224 |
| Origin |
NTIS |
| Type |
CAT |
