| Main Title |
Enzyme Immunoassay with Monoclonal Antibodies for the Detection of Rotavirus in Stool Specimens. |
| Author |
Herrmann, J. E. ;
Blacklow, N. R. ;
Perron, D. M. ;
Cukor, G. ;
Krause, P. J. ;
|
| CORP Author |
Massachusetts Univ. Medical School, Worcester.;Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1985 |
| Report Number |
EPA-R-810803; EPA/600/J-85/280; |
| Stock Number |
PB86-162294 |
| Additional Subjects |
Antibodies ;
Viruses ;
Diagnosis ;
Viral diseases ;
Feces ;
Humans ;
Reprints ;
Enzyme immunoassay
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB86-162294 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
5p |
| Abstract |
Rotavirus infections are generally recognized as a major problem in young children; however, they have also been associated with severe gastroenteritis in adults and neonates. Infections in neonates are usually asymptomatic, although the incidence of infection may be high. Adult infections also are often inapparent or mild, and thus, adults may be a reservoir of infection in pediatric wards. Diagnosis of infection is now routinely performed by enzyme immunoassay (EIA) in preference to the more cumbersome electron microscopy (EM). A commercial polyclonal EIA test, Rotazyme (Abbott Laboratories, North Chicago, Ill.), has been widely used for this purpose. The efficacy of this assay in adults has not been described, and reports of false-positive reactions in samples from neonates have resulted in recommendations that Rotazyme not be used with neonatal stool specimens. False-positive results in neonates have also been reported for other rotaviral EIA tests based on polyclonal sera. |
| Supplementary Notes |
Pub. in Jnl. of Infectious Diseases, v152 n4 p830-832 Oct 85. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Enzyme Immunoassay with Monoclonal Antibodies for the Detection of Rotavirus in Stool Specimens. |
| PUB Date Free Form |
c1985 |
| Category Codes |
6E; 6M; 57E; 57K |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
611417706 |
| Origin |
NTIS |
| Type |
CAT |
