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Main Title Modulation of eicosanoid production by human alveolar macrophases exposed to silica 'in vitro'. {microfiche}
Author Koren, H.S. ; Joyce, M. ; Devlin, R. B. ; Becker, S. ; Driscoll., K.
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. School of Medicine. ;Procter and Gamble Co., Cincinnati, OH. Miami Valley Labs. ;ABB Environmental, Inc., Chapel Hill, NC.
Publisher US Environmental Protection Agency, Health Affects Research Laboratory,
Year Published 1991
Report Number EPA/600/D-90/196
Stock Number PB91-136630
Additional Subjects Silicon organic compounds ; Toxicity ; Dust ; Particles ; Respiration ; In vitro analysis ; Liquid chromatography ; Inflammation ; Lung ; Humans ; Laboratory animals ; Pulmonary alveoli ; Macrophages ; Eicosanoids ; Dose-response relationships ; Bronchoalveolar lavage fluid ; Lipoxygenases ; Prostaglandin synthase ; Cell survival ; Leukotrienes ; Radioimmunoassay
Library Call Number Additional Info Location Last
NTIS  PB91-136630 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 30 p. : ill. ; 29 cm.
Abstract The objective of the study was to determine the eicosanoid production by human alveolar macrophages in response to silica exposure in vitro and to assess their contribution to silica-induced fibrosis and inflammation. Macrophages were obtained from healthy volunteers and were incubated for 3 or 24 hours in the presence of silica (100, 60, and 0 microgram/ml). Supernatants were removed for eicosanoid analysis. Eicosanoids were analyzed by both HPLC and RIA. The data suggested that silica caused an increased release of LTB4, LTC4/D4/E4, and 5-HETE after 3 hours; and decreases in PGE2 and TXB2 production after 24 hours exposure to 100 microgram/ml silica. In addition, 12-HETE and 15-HETE production remained unchanged at either time point. These opposing effects seen with the metabolites of lipoxygenase and cyclooxygenase pathways could contribute to silica-induced fibrosis. The pattern of eicosanoid production after exposure to silica was different from that obtained when macrophages were stimulated with LPS for 3 or 24 hrs, indicating that the response to the particles was not just due to general cellular activation.
Notes "EPA 600/D-90/196."
Place Published Research Triangle Park, NC.,
Supplementary Notes Prepared in cooperation with North Carolina Univ. at Chapel Hill. School of Medicine, Procter and Gamble Co., Cincinnati, OH. Miami Valley Labs., and ABB Environmental, Inc., Chapel Hill, NC.
PUB Date Free Form 1990.
NTIS Prices PC A03/MF A01
BIB Level m
Cataloging Source OCLC/T
OCLC Time Stamp 20001226165342
Language eng
Origin NTIS
OCLC Rec Leader 00813nam 2200205Ka 45020