Full Record Display for the EPA National Library Catalog

RECORD NUMBER: 3 OF 3

Main Title

The dynamics of an estuary as a natural ecosystem, II /

Author

Vernberg, F. John, ; Koch, Robert B. ; Kitchens, W. ; McKellar, H. ; Summers, K. ; Bonnell, R.

Other Authors

Author	Title of a Work
Kitchens, Wiley M.,
McKellar, H. N.
Summers, K.,
Bonnell, R.,
Vernberg, F. John,

CORP Author

Mississippi State Univ., Mississippi State. Dept. of Biochemistry.;Environmental Research Lab., Gulf Breeze, FL.

Publisher

U.S. Environmental Protection Agency, Office of Research and Development, Environmental Research Laboratory,

Year Published

1978

Report Number

EPA-600/3-78-092; EPA-R-804407-01

Stock Number

PB-289 969

OCLC Number

08081667

Subjects

Estuarine ecology--South Carolina ; Estuarine ecology--Mathematical models ; Salt marsh ecology--South Carolina ; Salt marsh ecology--Mathematical models ; Tidemarsh ecology--South Carolina ; Tidemarsh ecology--Mathematical models

Additional Subjects

Pesticides ; Antibodies ; Development ; Chlorine organic compounds ; Inhibition ; Recommendations ; Rabbits ; Chickens ; Laboratory animals ; Antigen antibody reactions ; Enzymes ; Insecticides ; Chlorine organic compounds ; Kelevan ; Adenosine triphosphatase ; Kepone ; Immune serums ; Methenocyclobutapentalene levulinic acid/decachlorooctahydro-hydroxy-(ethyl-ester) ; Cyclobutapentalenone/decachlorocathydro ; North Inlet Estuary ; Ecology ; Water pollution ; Mathematical models ; Aquatic plants ; Aquatic animals ; Plankton ; Intertidal zone ; Marine microorganisms ; Invertebrates ; Food chains ; Primary biological productivity ; Fishes ; Birds ; Respiration ; Biomass ; Nutrients ; Computer programming ; South Carolina ; Spartina alterniflora ; Salt marshes ; Baseline studies

Internet Access

Description	Access URL
	https://nepis.epa.gov/Exe/ZyPDF.cgi?Dockey=9101XGX1.PDF
	https://nepis.epa.gov/Exe/ZyPDF.cgi?Dockey=9101XGZG.PDF

Holdings

Library	Call Number	Additional Info	Location	Last Modified
EJBD	EPA 600-3-78-092	c.1	Headquarters Library/Washington,DC	03/24/2014
EJDD	EPA 600/3-78-092		Env Science Center Library/Ft Meade,MD	03/31/1995
EKCD	EPA-600/3-78-092		CEMM/GEMMD Library/Gulf Breeze,FL	07/21/2015
ELBD ARCHIVE	EPA 600-3-78-092	Received from HQ	AWBERC Library/Cincinnati,OH	10/04/2023
ERAD	EPA 600/3-78-092		Region 9 Library/San Francisco,CA	10/22/2012
ESAD	EPA 600-3-78-092		Region 10 Library/Seattle,WA	08/23/2017
NTIS	PB-289 963	Some EPA libraries have a fiche copy filed under the call number shown.		07/26/2022
NTIS	PB-289 969	Some EPA libraries have a fiche copy filed under the call number shown.		07/26/2022

Collation

viii, 76 pages : illustrations ; 28 cm.

Abstract

The report describes development of an antibody to an organochlorine pesticide to be used in studies related to its inhibition of the ATPase system. Kelevan, the condensation product of ethyl levulinate and Kepone, was successfully conjugated to bovine serum albumin (BSA), fibrinogen (BF), and gamma globulin (BGG). Rabbits and chickens preimmunized with BSA and then immunized with BSA-Kelevan produced antibodies to both the hapten, Kelevan, and the carrier protein BSA. Antiserum to Kelevan protected ATPase activity against Kepone and its derivatives. The titer of antibody to Kelevan was critical since antiserum with only trace amounts of Kelevan antibody failed to protect the ATPase activity against Kepone Inhibition. Antibody was concentrated by Na2S04 fractional precipitation of the antiserum and obtained in pure form by affinity chromatography using BGG-Kel covalently linked to Sepharose 4B. Pure antibody was obtained from untreated blood serum or plasma with no prior pretreatment or fractionation using the BGG-Kel affinity column. Complete protection of mitochondrial mg2+ATPase activity from in vitro inhibition of Kepone was obtained using a 1.2 mg quantity of na2S04 fractionated antibody and only 120 micrograms of pure antibody. Reversal of ATPase inhibition was readily obtained by addition of antibody prior to addition of substrate to the reaction mixture.

Notes

"Belle W. Baruch Institute for Marine Biology and Coastal Research, University of South Carolina." "October 1978." Microfiche Includes bibliographical references (pages 72-76).

Place Published

Gulf Breeze, Fla. Springfield, Va.

Supplementary Notes