||Relative Concentrations of Serum Neutralizing Antibody to VP3 and VP7 Proteins in Adults Infected with a Human Rotavirus (Journal Version).
Ward, R. L. ;
Knowlton, D. R. ;
Schiff, G. M. ;
Hoshino, Y. ;
Greenberg, H. B. ;
||James N. Gamble Inst. of Medical Research, Cincinnati, OH. ;Veterans Administration Medical Center, Palo Alto, CA. ;National Institutes of Health, Bethesda, MD.;Health Effects Research Lab., Research Triangle Park, NC.
Viral proteins ;
Antibody formation ;
Neutralization tests ;
Genetic recombination ;
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||Two outer capsid rotavirus proteins, VP3 and VP7, have been found to elicit neutralizing antibody production, but the immunogenicity of these proteins during human rotavirus infection has not been determined. The relative amounts of serum neutralizing antibody against the VP3 and VP7 proteins of the CJN strain of human rotavirus were, therefore, determined in adults subjects before and after infection with this virus. Reassortant strains of rotavirus that contained the CJN gene segment for only one of these two neutralization proteins were isolated and used. The geometric mean titer of serum neutralizing antibody to a reassortant virus (CJN-M) that contained VP7 of CJN and VP3 of another human rotavirus was 12.7 times less than that of antibody to CJN before infection and 20.3 times less after infection. This indicated that most neutralizing antibody was against the CP3 rather than the VP7 protein of CJN. The result was confirmed with other reassortants between CJN and animal rotavirus strains (EDIM and rhesus rotavirus). The findings suggest that CP3 is the primary immunogen that stimulates neutralizing antibody during at least some rotavirus infections of humans. (Copyright (c) 1988 American Society for Microbiology.)
||Pub. in Jnl. of Virology, v62 n5 p1543-1549 May 88. Prepared in cooperation with Veterans Administration Medical Center, Palo Alto, CA., and National Institutes of Health, Bethesda, MD. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
|NTIS Title Notes
||Reprint: Relative Concentrations of Serum Neutralizing Antibody to VP3 and VP7 Proteins in Adults Infected with a Human Rotavirus (Journal Version).
||Not available NTIS