| Main Title |
Nitropyrene: DNA Binding and Adduct Formation in Respiratory Tissues. |
| Author |
Jackson, M. A. ;
King, L. C. ;
Ball, L. M. ;
Ghayourmanesh, S. ;
Jeffrey, A. M. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering. ;Columbia Univ. Health Science Center, New York. |
| Year Published |
1985 |
| Report Number |
EPA/600/J-85/301; |
| Stock Number |
PB86-162336 |
| Additional Subjects |
Toxicology ;
Deoxyribonucleic acids ;
Tissues(Biology) ;
Binding ;
Lung ;
Reprints ;
Nitropyrene ;
Metabolites
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB86-162336 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
7p |
| Abstract |
Binding of 1-nitro(14C)pyrene (NP) or its metabolites to cellular DNA and protein in cultures of rabbit alveolar macrophages, lung tissue and tracheal tissue was examined. DNA binding in tracheal tissue (136 + or - 18.3 pmol NP/mg DNA) was four to five times the levels measured in either lung tissue (38 + or - 9.4 pmol NP/mg DNA) or macrophages (26 + or - 7.5-pmol NP/mg DNA). Adduct analysis of DNA isolated from lung tissue incubated with 3H-1-nitropyrene in vitro resulted in the identification of 2-5% of the NP adducts as C8-deoxyguanosine 1-aminopyrene. NP was also bound to cellular protein in tracheal tissue and lung tissue, and at a lower level in macrophages. Co-cultivation of the macrophages with lung and tracheal tissue decreased the DNA binding in tracheal tissue by 45%. Following intratracheal instillation of diesel particles (5 mg) vapor-coated with 14C-NP (380 ppm, 0.085 microCi/mg) particles into rats, 5 to 8% of the radioactivity remained in the lungs after 20 hours. Most of the diesel particles were also deposited in the lung. Examination of DNA and protein binding in this tissue showed 5-12% of the pulmonary 14C bound to protein and no detectable levels of 14C bound to DNA. |
| Supplementary Notes |
Prepared in cooperation with Environmental Health Research and Testing, Inc., Research Triangle Park, NC., North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering, and Columbia Univ. Health Science Center, New York. |
| Availability Notes |
Pub. in Environmental Health Perspectives, v62 p203-207 Oct 85. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Nitropyrene: DNA Binding and Adduct Formation in Respiratory Tissues. |
| PUB Date Free Form |
1985 |
| Category Codes |
6T; 6E; 57Y; 57O |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
612915195 |
| Origin |
NTIS |
| Type |
CAT |
