||Embryonic Development in vitro Following Short-Duration Exposure to Heat.
Kimmel, G. L. ;
Cuff, J. M. ;
Kimmel, C. A. ;
Heredia, D. J. ;
Tudor, N. ;
||Environmental Protection Agency, Washington, DC. Office of Health and Environmental Assessment. ;Thiel Coll., Greenville, PA. Dept. of Biology. ;Food and Drug Administration, Rockville, MD. Center for Devices and Radiological Health.
Embryo development ;
Induced hyperthermia ;
Body temperature ;
In vitro analysis ;
Animal pregnancy ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Gestation day (GD) 10 rat embryos (10-12 somites) were exposed in vitro for 10 to 25 mins at 42 C or 43 C and evaluated 24 hrs later for alterations in growth and specific morphological parameters, using a modified Brown-Fabro scoring system that allowed evaluation of development relative to gestational age. At 42 C, crown-rump length appeared to be particularly sensitive, responding to only 10 mins exposure. A 15-min exposure resulted in decreased total protein, somite number and morphological score. At 43 C, all of the parameters measured were affected by a 10-min exposure. The response patterns demonstrated a clear temperature- and exposure duration-dependency, with a shift from a more shallow duration-response curve to a more dramatic inhibition of development as temperature increased from 42 C to 43 C.
||Pub. in Teratology 47, p243-251 Feb 93. Prepared in cooperation with Thiel Coll., Greenville, PA. Dept. of Biology, and Food and Drug Administration, Rockville, MD. Center for Devices and Radiological Health.
|NTIS Title Notes
||Reprint: Embryonic Development in vitro Following Short-Duration Exposure to Heat.
||PC A03/MF A01