Full Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Endotoxins, Algae and 'Limulus' Amoebocyte Lysate Test in Drinking Water.
Author Sykora, Jan L. ; Keleti, Georg ; Roche, Richard ; Volk, David R. ; Kay, George P. ;
CORP Author North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.;Health Effects Research Lab., Cincinnati, OH.
Year Published 1980
Report Number EPA-R-805368; EPA-600/J-80-199;
Stock Number PB81-136525
Additional Subjects Algae ; Bacteria ; Potable water ; Lipopolysaccharides ; Distribution ; Surveys ; Pennsylvania ; Phytoplankton ; Reprints ; Drinking water ; Chlorella vulgaris ; Schizothrix calcicola ; Limulus ; Allegheny County(Pennsylvania)
Library Call Number Additional Info Location Last
NTIS  PB81-136525 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 13p
Abstract Field and laboratory studies were conducted to determine the distribution of algae and bacteria, and investigate sources of endotoxins (lipopolysaccharides) in drinking water. The field survey was performed on five drinking water systems located in Allegheny County, Pennsylvania during the spring and summer of 1978. The highest concentrations of phytoplankton were found in uncovered finished water reservoirs. The major source of 'endotoxic' response as measured by the Limulus amoebocyte lysate (LAL) gelation test was a non-specific reaction caused by algae. This was documented by a highly positive correlation of phytoplankton concentrations occurring in the reservoirs with respective LAL titers. Chlorella vulgaris (Chlorophyta) was the most common alga, whereas Schizothrix calcicola was the most dominant Cyanobacterium found in the five water systems. LAL gelation with C. vulgaris grown in the laboratory verified the phenomenon observed on samples collected in the field and indicated a non-specific reaction, whereas S. calcicola cultures under identical conditions produced a specific response. Alkali and lysozyme treatments were successful in distinguishing specific and non-specific LAL reactions. These two techniques in conjunction with LAL test are recommended for drinking water quality assessment.
Supplementary Notes Pub. in Water Research, v14 p829-839 1980.
NTIS Title Notes Journal article.
Title Annotations Reprint: Endotoxins, Algae and 'Limulus' Amoebocyte Lysate Test in Drinking Water.
PUB Date Free Form 1980
Category Codes 6F; 6M; 68D; 57K
NTIS Prices PC A02/MF A01
Primary Description 600/10
Document Type NT
Cataloging Source NTIS/MT
Control Number 323790634
Origin NTIS
Type CAT