||Using the DNA Alkaline Unwinding Assay to Detect DNA Damage in Laboratory and Environmentally Exposed Cells and Tissues.
Nacci, D. ;
Jackim., E. ;
||Science Applications International Corp., Narragansett, RI.;Environmental Research Lab., Narragansett, RI.
||EPA/600/J-89/419 ; ERLN-1033
Marine biology ;
In vitro analysis ;
DNA unwinding proteins ;
Water pollution effects(Animals) ;
DNA damage ;
Mutagenicity tests ;
Dose-response relationships ;
Polychlorobiphenyl compounds ;
Alkylating agents ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||The DNA alkaline unwinding assay is being evaluated for use in the detection of DNA damage in marine animals exposed to environmental pollutants. In preliminary work, DNA unwinding methods were used with in vitro cell systems to demonstrate DNA strand breaks. Cultured mammalian fibroblasts and sperm from marine fish and invertebrates (Pseudopleuronectes, Arbacia and Mytilus) showed concentration-dependent increases in DNA strand breaks after brief exposures to alkylating agents. DNA unwinding methods were also used on DNA extracted from marine animals injected with genotoxicants and from animals exposed in situ at an estuarine site. Gills from blue mussels caged at the New Bedford Harbor Superfund Site (MA, USA) highly contaminated with many organic (e.g. PCBs) and inorganic contaminants, were also examined. A significant increase in DNA strand breaks was seen in gill tissues of animals held in the contaminated site for as little as 3 days. Although not as severe, an increase in strand breaks was also seen in animals held at the control site for 28 days. (Copyright (c) 1990, Elsevier Science Publishers Ltd., England.)
||Pub. in Marine Environmental Research 28, p333-337 1989. Sponsored by Environmental Research Lab., Narragansett, RI.
|NTIS Title Notes
||Reprint: Using the DNA Alkaline Unwinding Assay to Detect DNA Damage in Laboratory and Environmentally Exposed Cells and Tissues.
||68D; 57Y; 57F; 47D
||PC A02/MF A01