| Main Title |
Preparation of Biological Tissue Sections for Correlative Ion, Electron, and Light Microscopy. |
| Author |
Kupke, K. G. ;
Pickett, J. P. ;
Ingram, P. ;
Griffis, D. P. ;
Linton, R. W. ;
|
| CORP Author |
Duke Univ. Medical Center, Durham, NC. ;Veterans Administration Medical Center, Durham, NC. ;Research Triangle Inst., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. Dept. of Chemistry.;Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1984 |
| Report Number |
EPA-R-807560; EPA/600/J-84/354; |
| Stock Number |
PB86-136223 |
| Additional Subjects |
Electron microscopy ;
Microscopy ;
Tissues(Biology) ;
Reprints ;
Ion microscopy
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB86-136223 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
12p |
| Abstract |
In order to correctly interpret the chemical images obtained using ion microscopy (IM), it is useful to correlate them with the information provided by conventional light microscopy (LM), secondary electron imaging (SEI), backscattered electron imaging (BEI), and electron probe microanalysis (EPMA). Accordingly, the authors have devised a technique of specimen preparation which allows for the application of several different microanalytical techniques to a single histologic section mounted on the same substrate. Sections are cut onto polyester plastic coverslips (devoid of peaks for any element with atomic number >9 using EPMA) and studied by LM. After a light rotary coating with carbon (to prevent charging), the section can then be examined by SEI, BEI, and EPMA. Specific areas can be marked for IM study either with an objective-mounted pin tissue microlocater, or by placing small pieces of metal foil, cut in specific geometric shapes, over features of interest. |
| Supplementary Notes |
Prepared in cooperation with Veterans Administration Medical Center, Durham, NC., Research Triangle Inst., Research Triangle Park, NC., and North Carolina Univ. at Chapel Hill. Dept. of Chemistry. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| Availability Notes |
Pub. in Jnl. of Electron Microscopy Technique 1, p299-309 1984. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Preparation of Biological Tissue Sections for Correlative Ion, Electron, and Light Microscopy. |
| PUB Date Free Form |
c1984 |
| Category Codes |
6C; 57F |
| NTIS Prices |
Not available NTIS |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
607218368 |
| Origin |
NTIS |
| Type |
CAT |
