| Main Title |
Enteroviruses in Sludge: Multiyear Experience with Four Wastewater Treatment Plants. |
| Author |
Hamparian, V. V. ;
Ottolenghi, A. C. ;
Hughes, J. H. ;
|
| CORP Author |
Ohio State Univ., Columbus.;Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1985 |
| Report Number |
EPA-R-805189; EPA/600/J-85/239; |
| Stock Number |
PB86-137007 |
| Additional Subjects |
Enteroviruses ;
Sludge ;
Viruses ;
Isolation ;
Reprints ;
Sewage treatment
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB86-137007 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
8p |
| Abstract |
The authors describe their experience with the isolation of viruses from four treatment plants located in different geographic areas. Over a period of 3 years, 297 enteroviruses were isolated from 307 sludge samples. The highest frequency of viral isolation (92%), including multiple isolates from single samples, was obtained from a treatment plant serving the smallest population. Excluding the polioviruses, 22 different enterovirus serotypes were isolated. The methods used to isolate the viruses were relatively simple and included an elution procedure in which beef extract was use and a disinfection step. No concentration procedure was used. Of three cell culture systems used, the RD line of human rhabdomyosarcoma cells was by far the most useful for the Isolation of echoviruses; BGM and HeLa cells were particularly useful for the isolation of group B coxsackleviruses. A seasonal effect on viral isolation rates from sludge was observed. |
| Supplementary Notes |
Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| Availability Notes |
Pub. in Applied and Environmental Microbiology, v50 n2 p280-286 Aug 85. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Enteroviruses in Sludge: Multiyear Experience with Four Wastewater Treatment Plants. |
| PUB Date Free Form |
c1985 |
| Category Codes |
13B; 6M; 68D; 57K |
| NTIS Prices |
Not available NTIS |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
605827043 |
| Origin |
NTIS |
| Type |
CAT |
