Main Title |
Immunoblot Analysis of Exospore Polypeptides from Some Entomophilic Microsporidia. |
Author |
Irby, W. S. ;
Huang, Y. S. ;
Kawanishi, C. Y. ;
Brooks, W. M. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;North Carolina State Univ. at Raleigh. |
Year Published |
1986 |
Report Number |
EPA/600/J-86/052; |
Stock Number |
PB86-201506 |
Additional Subjects |
Exposures ;
Polypeptides ;
Immunology ;
Reprints ;
Microsporidia ;
Immunoblot analysis
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB86-201506 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
10p |
Abstract |
Immunological relationships between genera and species of microsporidia were examined by immunoblot analysis. Exospore polypeptides from 2 Nosema spp., 3 Vairimorpha spp., and 2 undescribed Vairimorpha-like spp. were analyzed. Gel electrophoresis and immunoblot analysis revealed that a variety of polypeptides, most between 15,000 and 90,000 molecular weight, were present on the exospore. The 3 Vairimorpha spp. were highly related immunologically to each other, but less so to the 2 Vairimorphalike undescribed spp. The 2 Nosema spp. immunologically distant from each other and from the Vairimorpha spp. Indirect evidence, however, indicated that many internal spore polypeptides present in both genera are similar. Cross-reactivity between exospore polypeptides from entomophilic microsporidia and antisera to a mammalian microsporidium, Encephalitozoon cuniculi was very limited. |
Supplementary Notes |
Pub. in Jnl. Protozool. 33(1), n1 p14-20 Jan 86. Prepared in cooperation with North Carolina State Univ. at Raleigh. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Immunoblot Analysis of Exospore Polypeptides from Some Entomophilic Microsporidia. |
PUB Date Free Form |
c1986 |
Category Codes |
6E; 6M; 57J; 57K |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
621322914 |
Origin |
NTIS |
Type |
CAT |