||High Resolution Cytogenetic Characterization of the L5178Y TK+/- Mouse Lymphoma Cell Line.
Sawyer, J. R. ;
Hoyle, K. M. ;
Moore, M. M. ;
Hozier, J. C. ;
||Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.;Florida Inst. of Tech., Melbourne. Medical Genetics Lab.
Chromosome aberrations ;
Thymidine kinase ;
Cultured tumor cells ;
Acridine orange ;
Chromosome banding ;
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||High resolution chromosome preparations from L5178Y TK+/- 3.7.2C mouse lymphoma cells were obtained by the use of acridine orange in the cell harvest procedure. With this technique it is possible to visualize over 500 bands in elongated mouse lymphoma chromosomes as compared to the approximately 230 bands visualized in metaphase preparations. High resolution mouse lymphoma chromosomes are described and chromosome rearrangements carried in the cell line are characterized by ideograms, which represent the position, number, size, and relative staining intensity of the G-band patterns. Use of elongated chromosomes of mouse lymphoma TK+/- mutants should facilitate analysis of cytogenetic effects associated with TK+/- -> TK-/- mutagenesis. (Copyright (c) 1989 Elsevier Science Publishers B.V. (Biomedical Division).)
||Pub. in Mutation Research, v214 p181-193 Jun 89. Sponsored by Florida Inst. of Tech., Melbourne. Medical Genetics Lab.
|NTIS Title Notes
||Reprint: High Resolution Cytogenetic Characterization of the L5178Y TK+/- Mouse Lymphoma Cell Line.
||PC A03/MF A01