Main Title |
Influence of 50-Hz Electric and Magnetic Fields on Neurite Outgrowth in Pheochromocytoma Cells. |
Author |
Blackman, C. ;
Benane, S. ;
House, D. ;
Pollock, M. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC.;Department of Energy, Washington, DC. |
Publisher |
1991 |
Year Published |
1991 |
Report Number |
EPA/600/D-91/225; DE-AI01-89CE34024; |
Stock Number |
PB91-242461 |
Additional Subjects |
Pheochromocytoma ;
Electric fields ;
Magnetic fields ;
Nerves ;
Nerve growth factor ;
Cultured tumor cells ;
Growth ;
Adrenal medulla ;
Binding sites ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB91-242461 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
10p |
Abstract |
The study describes the ability of electric and magnetic fields to substitute for nerve growth factor in the stimulation of neurite outgrowth in a subline of PC-12 cells, derived from a pheochromocytoma in rat adrenal medulla. The cells were plated on collagen-coated, 60-mm plastic petri dishes and exposed to sinusoidal 50-Hz magnetic fields for 22 hours in a 5% CO2 incubator at 37 C. Two 1000-turn coils, 20 cm in diameter, were assembled in a Helmholtz configuration to generate a magnetic field in a vertical orientation, thereby inducing a companion electric field. A co-netic metal shield housed the control samples in the same incubator. Total cells and number of cells with neurites at least as long as the cell diameter or having a growth cone were counted within an annulus of 1.7 to 1.8 cm radii. Sham exposure demonstrated no difference in % of cells with neurites between the exposed and control locations in the incubator. Exposures were done at 420, 230, 120, 42, 31, 22, 9 and 4.2 milliGauss (mG). At the dish radius of 1.7-1.8 cm these flux densities induced electric fields of 115, 63, 33, 12, 8.5, 6.0, 2.5 and 1.2 microV/m, respectively. At each field strength, there were two control dishes and six exposed dishes, except for 5 exposure dishes at 120 mG. The results demonstrate that electric and magnetic fields stimulate neurite outgrowth in a field strength-dependent manner between 22 and 42 mG (6.0 and 12 microV/m), reaching an apparent stimulation plateau between 42 and 420 mG(12 and 115 microV/m). |
Supplementary Notes |
Sponsored by Department of Energy, Washington, DC. |
Category Codes |
57V; 57F; 57I |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
131121640 |
Origin |
NTIS |
Type |
CAT |