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Main Title Interlaboratory Study of Cellular Fluorescence Intensity Measurements with Fluorescein-Labeled Microbead Standards.
Author Vogt, R. F. ; Cross, G. D. ; Phillips, D. L. ; Henderson, L. O. ; Hannon., W. H. ;
CORP Author Corvallis Environmental Research Lab., OR. ;Centers for Disease Control, Atlanta, GA.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-92/096;
Stock Number PB92-153881
Additional Subjects Interlaboratory comparisons ; Fluorescence ; Cells(Biology) ; Fluoresceins ; T4 lymphocytes ; Flow cytometry ; Quality control ; Humans ; Reprints ; Microbeads
Library Call Number Additional Info Location Last
NTIS  PB92-153881 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
Abstract To determine the precision of cellular fluorescence intensity (FI) measurements derived from labeled microbead standards, FI results were compared from 43 different flow cytometers in 34 laboratories. All laboratories analyzed prepared aliquots of fluoresceinated calf thymocyte nuclei (Fluorotrol), human lymphocytes stained with fluoresceinated anti-CD4 antibody, and fluoresceinated microbeads used as both internal and external standards. Results for percent of events within the gates and the histograms returned by participants indicated that the samples had remained stable and that gated populations had been properly identified. All standard curves showed strong linearity, and the pooled results from all standards produced a best-fit curve that was in close agreement with the assigned values. Nonetheless, results for cellular FI were highly variable, with CVs of 20-34%. Agreement within lab/instrument was much better, with CVs ranging from 3.0 to 9.9%. The overall variability was not obviously attributable to differences in the types of cytometer, nor could it be explained by attributes of the standard curves or any other single variable examined.
Supplementary Notes Pub. in Cytometry, v12 p525-536 1991. Prepared in cooperation with Centers for Disease Control, Atlanta, GA.
NTIS Title Notes Journal article.
Title Annotations Reprint: Interlaboratory Study of Cellular Fluorescence Intensity Measurements with Fluorescein-Labeled Microbead Standards.
Category Codes 57F
NTIS Prices PC A03/MF A01
Primary Description 600/02
Document Type NT
Cataloging Source NTIS/MT
Control Number 216422850
Origin NTIS
Type CAT