| Main Title |
Reaction of Cyclopent(c,d)pyrene-3,4-epoxide with DNA and Deoxynucleotides. |
| Author |
Beach, A. C. ;
Agarwall, S. A. ;
Lambert, G. R. ;
Nesnow, S. ;
Gupta, R. C. ;
|
| CORP Author |
Kentucky Univ., Lexington. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. |
| Publisher |
c1993 |
| Year Published |
1993 |
| Report Number |
EPA-68-D1-0148; EPA/600/J-93/337; |
| Stock Number |
PB93-229557 |
| Additional Subjects |
Deoxyribonucleic acids ;
Mutagens ;
Carcinogens ;
Nucleotides ;
Microsomes ;
DNA damage ;
Reprints ;
Epoxide/cyclopentapyrene
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-229557 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
7p |
| Abstract |
Cyclopenta(c,d)pyrene (CPP) is a ubiquitous polycyclic aromatic hydrocarbon with potent mutagenic and carcinogenic activity. The trans isomer of 3,4-dihydro-3,4-dihydroxy-cyclopenta(c,d)pyrene has been shown to be the major metabolic product of CPP in rat, mouse, or human microsomal systems as well as in peroxyl radical-generating systems indicating the preferential formation of its obligatory precursor, CPP-3,4-epoxide. The direct mutagenicity of CPP-3,4-epoxide, the inactivity of 3,4-dihydro-CPP, and the DNA adduct forming capacity of CPP in vivo has prompted analysis of the DNA adduct forming capacity of CPP-3,4-epoxide to provide information pertaining to: (1) the role this postulated major ultimate mutagenic metabolite may plan in the formation of DNA adducts in vivo; (2) the base selectivity of CPP-3,4-epoxide DNA adducts; and (3) the role of CPP-3,4-epoxide in the mutagenicity/carcinogenicity of CPP. CPP-3,4-epoxide was reacted with calf thymus DNA, dGp, dAp, dTp, dCp, poly dB-dC, poly dA-dT, and poly dG. Adducts were analyzed by the butanol-enhanced version of (32)P-postlabeling. Four major and at least three minor adducts formed with DNA in vitro, which were further analyzed for their base-selectivity. A similar spectra of adducts was exhibited by dGp, poly dG-dC and poly dG. dCp, dTp, and dAp formed one, two, and four adducts, respectively. Adducts derived from either dGp, poly dG-dC, or poly dG comigrated with the DNA adducts in 3 solvent systems indicating that CPP-3,4-epoxide forms DNA adducts almost exclusively with deoxyguanosine. |
| Supplementary Notes |
Pub. in Jnl. of Carcinogenesis, v14 n4 p767-771 Apr 93. Prepared in cooperation with Environmental Health Research and Testing, Inc., Research Triangle Park, NC. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Reaction of Cyclopent(c,d)pyrene-3,4-epoxide with DNA and Deoxynucleotides. |
| Category Codes |
57Y; 57F |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
328023534 |
| Origin |
NTIS |
| Type |
CAT |
