Main Title |
Cellular Toxicity in Chinese Hamster Ovary Cell Cultures. 1. Analysis of Cytotoxicity Endpoints for Twenty-Nine Priority Pollutants. |
Author |
Garrett, N. E. ;
Lewtas, J. ;
|
CORP Author |
Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1983 |
Report Number |
EPA-68-02-2566; EPA-600/J-83-172; |
Stock Number |
PB84-173541 |
Additional Subjects |
Cell cultures ;
Hamsters ;
Exposure ;
Reprints ;
Toxic substances ;
Cytotoxicity
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB84-173541 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
13p |
Abstract |
Chinese hamster ovary cells were exposed to 29 toxic chemical substances which were representative of several classes of compounds listed by the Natural Resources Defense Council Consent Decree as priority toxic pollutants. After cell cultures were exposed to the test substance, cell samples were assayed for protein and DNA synthesis, ATP, cell number, and viability. A filter-disk technique employing a batch-washing procedure was used for the determination of protein and DNA synthesis. Dose-response data were obtained for 15 of the more toxic agents including chlorinated aromatics, metallic compounds phenols, and polychlorinated biphenyls. Estimates of the sample concentrations necessary to produce a 50% reduction in response were used to compare cytotoxicity endpoints. ATP and protein synthesis were approximately equally effective as indicators of cellular toxicity. Cadmium chloride, nickel nitrate, arsenic trioxide, and potassium chromate produced a more pronounced effect on DNA synthesis was a smooth, continuous function for responses as low as 1 to 2% of the control. |
Availability Notes |
Pub. in Environmental Research 32, p455-465 1983. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Cellular Toxicity in Chinese Hamster Ovary Cell Cultures. 1. Analysis of Cytotoxicity Endpoints for Twenty-Nine Priority Pollutants. |
PUB Date Free Form |
c1983 |
Category Codes |
6T; 57Y |
NTIS Prices |
Not available NTIS |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
417425174 |
Origin |
NTIS |
Type |
CAT |