| Main Title |
Identification of Immunogens of 'Mycoplasma pneumoniae' by Protein Blotting. |
| Author |
Hu, Ping C. ;
Huang, Yuan-shen ;
Graham, Judith A. ;
Gardner, Donald E. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1981 |
| Report Number |
EPA-600/J-82-029; |
| Stock Number |
PB83-125062 |
| Additional Subjects |
Bacteria ;
Protein ;
Immunology ;
Identifying ;
Electrophoresis ;
Hamsters ;
Rabbits ;
Antibodies ;
Reprints ;
Mycoplasma pneumoniae ;
Blotting ;
Immunogens
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB83-125062 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
Proteins of Mycoplasma pneumoniae were separated by SDS-polyacrylamide gel electrophoresis and transferred to a nitrocellulose sheet by blotting. Sera obtained from infected hamsters and immunized rabbits were then incubated with the nitrocellulose strips. Proteins which are capable of eliciting antibodies were detected by indirect immunoradioautography using 125I-labeled antisera against hamster or rabbit IgG. Antibodies to seven immunogens were demonstrated in the sear of hamsters infected with M. pneumoniae by inhalation, while many more proteins were found to be capable of stimulating antibodies in rabbits immunized parenterally with mycoplasmas. |
| Supplementary Notes |
Pub. in Biochemical and Biophysical Research Communications, v103 n4 p1363-1370 1981. Prepared in cooperation with Northrop Services, Inc.-Environmental Sciences, Research Triangle Park, NC. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Identification of Immunogens of 'Mycoplasma pneumoniae' by Protein Blotting. |
| PUB Date Free Form |
4 Nov 81 |
| Category Codes |
6E; 57J |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
306319560 |
| Origin |
NTIS |
| Type |
CAT |
