Main Title |
In vitro toxicity indicators |
Other Authors |
|
Publisher |
Academic Press, |
Year Published |
1994 |
OCLC Number |
30501005 |
ISBN |
0124612032 (hardcover : alk. paper) 0124612040 (papercover : alk. paper) |
Subjects |
Cells--drug effects ;
Cell Death ;
Biological Markers--analysis ;
Indicators (Biology) ;
Toxicity testing--In vitro
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
EHBM |
RA1190.M48 v.1,pt.B |
|
CEMM/ACESD Library/Narragansett,RI |
01/26/2001 |
|
Collation |
xx, 497 p. : ill. |
Notes |
Includes bibliographical references and index. |
Contents Notes |
Perspectives -- Cellular and molecular pathobiology of reversible and irreversible injury -- Molecular mechanisms of toxic cell death: an overview -- Membrane lipid peroxidation: cellular mechanisms and toxicological implications -- Membrane integrity and function -- Enzyme leakage as an indicator of cytoxicity in cultured cells -- Cellular K+ -- Morphological and biochemical investigations on plasma membrane blebbing during cell injury -- Morphological evaluation of gap junctional intercellular communication. Fluorescence microscopic examination of cytoskeletal elements -- Energy coupled Na+ cotransport -- Renal organic ion transport -- Endoplasmic reticulum calcium pump -- Energy metabolism -- Measurement of oxygen consumption -- ATP and other energetic parameters as indicators of cell injury -- Evaluation of glucose metabolism in vitro: altered competence of intermediary metabolism as an index of hepatotoxicity -- DNA-related indices -- Quantitation of DNA by fluorometry -- Techniques for measuring cell proliferation. Nuclease P1-enhanced 32P-postlabeling assay for aromatic carcinogen-DNA adducts -- Genomic hypomethylation -- DNA degradation: double-strand breaks -- Genotypic mutation analysis by RFLP/PCR -- Functional indices -- Gluconeogenesis in liver cells -- Measurement of protein synthesis -- Measuring collagen synthesis -- Measurement of macrophage phagocytosis using a sheep red blood cell assay -- Biochemical indices -- Cell function studies using fluorescent Ca2+ indicators -- Ca2+-bioluminescent indicators. Measurement of receptor-activated accumulation of inositol phosphates as an index of phospholipase C activity -- Covalent binding to proteins -- Glutathione, oxidized glutathione and mixed disulfides in biological samples -- Lipid peroxidation -- Thiobarbituric acid assay reactants -- Volatile hydrocarbons -- Electronically excited state formation -- Spectrophotometric detection of lipid-conjugated dienes -- Quantitation of conjugated dienes by a
dieles-alder reaction -- Quantitation of conjugated dienes by second-derivative UV spectroscopy. Specialized techniques -- Centrifuge filtration separation of cells and the measurement of constituents -- Flow cytometry -- Single-cell microscopic techniques for studying toxic injury -- Measurement of macrophage and neutrophil chemotaxis -- Two-dimensional electrophoresis as applied to problems of in vitro and in vivo toxicology.
|
Place Published |
San Diego : |
PUB Date Free Form |
c1994. |
Series Title Traced |
Methods in toxicology, v. 1B |
BIB Level |
m |
Cataloging Source |
OCLC/T |
OCLC Time Stamp |
20010124164756 |
Language |
eng |
Origin |
OCLC |
Type |
CAT |
OCLC Rec Leader |
03719cam 2200433 a 45020 |