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Main Title Determination of 'Giardia muris' Cyst Viability by Differential Interference Contrast, Phase, or Brightfield Microscopy.
Author Schupp, D. G. ; Erlandsen, S. L. ;
CORP Author Minnesota Univ., Minneapolis. Medical School.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1987
Report Number EPA/600/J-87/201;
Stock Number PB88-170725
Additional Subjects Microscopy ; Cysts ; Viability ; Reprints ; Differential interference contrast ; Giardia muris
Library Call Number Additional Info Location Last
NTIS  PB88-170725 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Abstract Recent experiments have demonstrated that fluorogenic substrates are taken up by Giardia cysts and that an excellent correlation exists between animal infectivity and vital staining with fluorescein diacetate (FDA) for viable cysts and propidium iodide (PI) for non-viable cysts. Examination of FDA stained Giardia muris cysts with either Nomarski differential interference contrast (DIC) or brightfield (BF) microscopy revealed a direct correlation between morphologic appearance and uptake of FDA or PI. Cysts incorporating FDA were all morphologically identical and exhibited (1) a clearly delineated cyst wall, (2) the presence of a distinct space between cyst wall and cytoplasm, and (3) flagella recognizable at one pole of the cyst. Examination of PI stained cysts demonstrated the presence of well-defined nuclei, intracellular axonemes, and curved elements of the adhesive disc. The cytoplasm of PI stained cysts contained a fine granular texture as opposed to the hyaline appearance of FDA stained cysts, and no space was observed separating the cyst wall from underlying cytoplasm in the PI cyst. The correlation of fluorogenic dye uptake by cysts and their morphologic appearance provides a simple, direct, and inexpensive method for determining the viability of Giardia muris cysts which should have wide applicability in studies using environmental factors or chemical agents that may effect Giardia cyst viability.
Supplementary Notes Pub. in Jnl. of Parasitology, v73 n4 p723-729 Aug 87. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Determination of 'Giardia muris' Cyst Viability by Differential Interference Contrast, Phase, or Brightfield Microscopy.
PUB Date Free Form c1987
Category Codes 57N; 57K
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 812722568
Origin NTIS
Type CAT