Main Title |
Mutagenesis of L5178Y/TK(+/-)-3.7.2C Mouse Lymphoma Cells by the Clastogen Ellipticine. |
Author |
Moore, M. M. ;
Brock, K. H. ;
Doerr, C. L. ;
DeMarini, D. M. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. |
Year Published |
1987 |
Report Number |
EPA/600/J-87/195; |
Stock Number |
PB88-165931 |
Additional Subjects |
Lymphoma ;
Mice ;
Reprints ;
Mutagenesis ;
Clastogen ellipticine
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB88-165931 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
13p |
Abstract |
The antitumor drug ellipticine is clastogenic in CHO cells (Bhuyan et al., 1972) but is only weakly mutagenic at the hprt locus in CHO cells (DeMarini et al., 1983; Singh and Gupta, 1983a,b). In the present study, the mutagenic and clastogenic activities of ellipticine were evaluated in L5178Y/TK+/- -3.7.2C mouse lymphoma cells. Unlike the results at the hprt locus, ellipticine is a potent mutagen at the tk locus, with as little as 50 ng/ml producing 270 TK-deficient mutants/10 to the 6th power survivors at approx. 50% survival (background = 72/10 to the 6th power survivors). This same dose of ellipticine induced 44 aberrations per 100 metaphases (background = 5/100 cells). At 400 ng/ml, ellipticine induced over 1,000 mutants/10 to the 6th power survivors at approx. 10% survival and produced 242 aberrations/100 cells. Most of the aberrations were chromosome rather than chromatid events. As expected for a compound acting primarily by a clastogenic mechanism, almost all of the TK-deficient mutants were small colonies. Thus, ellipticine is a potent clastogen in both Chinese hamster cells and in mouse lymphoma cells; however, it is a potent mutagen at only the tk locus and not at the hprt locus. |
Supplementary Notes |
Pub. in Environmental Mutagenesis, v9 p161-170 1987. Prepared in cooperation with Environmental Health Research and Testing, Inc., Research Triangle Park, NC. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Mutagenesis of L5178Y/TK(+/-)-3.7.2C Mouse Lymphoma Cells by the Clastogen Ellipticine. |
PUB Date Free Form |
c1987 |
Category Codes |
57Y; 57O |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
811323849 |
Origin |
NTIS |
Type |
CAT |