| Main Title |
Interspecies Cytogenetic Comparisons: Studies with X-Radiation and Bleomycin Sulfate. |
| Author |
Kligerman, A. D. ;
Bryant, M. F. ;
Doerr, C. L. ;
Halperin, E. C. ;
Kwanyuen, P. ;
|
| CORP Author |
Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Duke Univ. Medical Center, Durham, NC. Dept. of Radiation Oncology.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. |
| Publisher |
c1992 |
| Year Published |
1992 |
| Report Number |
EPA-68-02-4456; EPA/600/J-92/301; |
| Stock Number |
PB92-227438 |
| Additional Subjects |
Chromosome aberrations ;
Species diversity ;
Bleomycin ;
X rays ;
Comparison ;
In vitro analysis ;
Lymphocytes ;
Dose-response relationships ;
Humans ;
Rats ;
Mice ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB92-227438 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
11p |
| Abstract |
A series of in vitro experiments were conducted to determine if there are innate differences in the sensitivity of peripheral blood lymphocytes (PBLs) from different mammalian species to clastogens. Mouse, rat, and human whole blood samples were exposed to either 0, 0.38, 0.75, 1.5, or 3.0 Gy x-radiation or 0, 5, 10, 20, 40, or 80 micrograms/ml bleomycin for 4 h. Bromodeoxyuridine-containing cultures were initiated and the PBLs stimulated to divide with phytohemagglutinin. All cultures were harvested following a 3-h colcemid treatment. Slides were made and differentially stained, and first-division metaphases were scored for chromosome aberrations. In the x-radiation studies human PBLs were significantly more sensitive than mouse PBLs which were in turn more sensitive than rat PBLs as measured by either the total percent aberrant cells or the number of dicentrics. Results with bleomycin suggest that the mouse and human PBLs are equally sensitive to the clastogenic effects of bleomycin. These results indicate that there may be inherent differences in sensitivity among PBLs of mammalian species; however, more studies are needed to determine if the differences presented here hold for other agents. |
| Supplementary Notes |
Pub. in Environmental and Molecular Mutagenesis, v19 n3 p235-243 Apr 92. Prepared in cooperation with Duke Univ. Medical Center, Durham, NC. Dept. of Radiation Oncology. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Interspecies Cytogenetic Comparisons: Studies with X-Radiation and Bleomycin Sulfate. |
| Category Codes |
57Y; 57V; 57F |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
231126073 |
| Origin |
NTIS |
| Type |
CAT |
